About: Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug

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About: Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence- based CyQuant assay was employed as the readout method to analyse the cellular DNA content. The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence- based CyQuant assay was employed as the readout method to analyse the cellular DNA content. (en)
Title	Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug (en)
skos:prefLabel	Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug (en)
skos:notation	RIV/00216224:14110/14:00078212!RIV15-MSM-14110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(EE2.3.20.0185), P(EE2.3.30.0030)
http://linked.open...iv/cisloPeriodika	Suppl. 1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Samadder, Pounami Ovesná, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	52596
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/14:00078212
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cell line screen; drug treatment; liquid handling station; epMotion (R) (en)
http://linked.open.../riv/klicoveSlovo	drug treatment cell line screen epMotion (R) liquid handling station
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F617FCCA3A23]
http://linked.open...i/riv/nazevZdroje	Folia biologica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for analysis and modeling of tissues and organs Development of human resources in the field of cell biology St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Ovesná, Petra Suchánková, T. Souček, K. Samadder, Pounami
http://linked.open...ain/vavai/riv/wos	000343275800008
issn	0015-5500
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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