About: Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing radiation-induced MF in adapted hESCs exceeded the induced MF in non-adapted hESCs and differentiated cells. The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing radiation-induced MF in adapted hESCs exceeded the induced MF in non-adapted hESCs and differentiated cells. (en)
Title	Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts (en)
skos:prefLabel	Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts (en)
skos:notation	RIV/00216224:14110/14:00073651!RIV15-MSM-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(EE2.3.30.0009), P(GA13-19910S), P(GBP302/12/G157), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	20
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Salykin, Anton Hampl, Aleš Zerzánková, Lenka Pešl, Martin Rotrekl, Vladimír Krutá, Miriama Dvořák, Petr Raška, Jan Šeneklová, Monika Baumeisterová, Aneta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	31200
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/14:00073651
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mutation frequency; human embryonic stem cells; induced pluripotent stem cells; hypoxanthine phosphoribosyltransferase; base excision repair; apurinic/apyrimidinic endonuclease (en)
http://linked.open.../riv/klicoveSlovo	base excision repair human embryonic stem cells apurinic/apyrimidinic endonuclease hypoxanthine phosphoribosyltransferase mutation frequency induced pluripotent stem cells
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[68DE0550C59F]
http://linked.open...i/riv/nazevZdroje	Stem Cells and Development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	14 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions Employment of Newly Graduated Doctors of Science for Scientific Excellence Modeling DMD-related dilated cardiomyopathy using induced pluripotent stem cells-derived cardiomyocytes
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Bártová, Eva Dvořák, Petr Hampl, Aleš Zerzánková, Lenka Pešl, Martin Salykin, Anton Krutá, Miriama Raška, Jan Rotrekl, Vladimír Baumeisterová, Aneta Franek, Michal Košková, Stanislava Neelsen, Kai J. Šeneklová, Monika
http://linked.open...ain/vavai/riv/wos	000342614300004
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	1547-3287
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1089/scd.2013.0611
http://localhost/t...ganizacniJednotka	14110

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software