About: CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

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About: CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs00216-014-8053-8
Description	The effect of antibiotics on the microbial cells and concentration of antibiotics in the human body is essential for the effective use of antimicrobial therapy. The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric substances from nature. However, the determination of isoelectric points of ampholytic antibiotics by conventional techniques is time consuming. For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric points and pK (a)s, ampicillin (pI 4.9), ciprofloxacin (pI 7.4), ofloxacin (pI 7.1), tetracycline (pI 5.4), tigecycline (pI 9.7), and vancomycin (pI 8.1), were found and optimized in the suitable pH ranges pH 2.0-5.3, 2.0-9.6, and 9.0-10.4. The effect of antibiotics on the microbial cells and concentration of antibiotics in the human body is essential for the effective use of antimicrobial therapy. The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric substances from nature. However, the determination of isoelectric points of ampholytic antibiotics by conventional techniques is time consuming. For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric points and pK (a)s, ampicillin (pI 4.9), ciprofloxacin (pI 7.4), ofloxacin (pI 7.1), tetracycline (pI 5.4), tigecycline (pI 9.7), and vancomycin (pI 8.1), were found and optimized in the suitable pH ranges pH 2.0-5.3, 2.0-9.6, and 9.0-10.4. (en)
Title	CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices (en)
skos:prefLabel	CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices (en)
skos:notation	RIV/00216224:14110/14:00073246!RIV15-MV0-14110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(VG20112015021)
http://linked.open...iv/cisloPeriodika	25
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dvořáčková, Milada Holá, Veronika Růžička, Filip
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	7284
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/14:00073246
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capillary isoelectric focusing with UV detection; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Ampholytic antibiotics; Staphylococcus epidermidis; Human whole blood (en)
http://linked.open.../riv/klicoveSlovo	Staphylococcus epidermidis Ampholytic antibiotics Capillary isoelectric focusing with UV detection Human whole blood Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[60EF11020EA5]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical chemistry
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of instrumental methods for rapid detection and identification of biological agens in field samples
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	406
http://linked.open...iv/tvurceVysledku	Holá, Veronika Šlais, Karel Růžička, Filip Horká, Marie Kubesová, Anna Vykydalová, Marie Šalplachta, Jiří Dvořáčková, Milada
http://linked.open...ain/vavai/riv/wos	000342224300020
issn	1618-2642
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00216-014-8053-8
http://localhost/t...ganizacniJednotka	14110

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