About: Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter     Goto   Sponge   NotDistinct   Permalink

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Description
  • This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material – sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.
  • This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material – sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto. (en)
Title
  • Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter
  • Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter (en)
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  • Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter
  • Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter (en)
skos:notation
  • RIV/00216224:14110/14:00073211!RIV15-MV0-14110___
http://linked.open...avai/riv/aktivita
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  • I, P(VG20112015021)
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  • FEB
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  • 7790
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  • RIV/00216224:14110/14:00073211
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  • Candida parapsilosis group; biofilm; micropreparative solution isoelectric focusing; high-performance liquid chromatography; capillary isoelectric focusing; sonicate from vascular catheter (en)
http://linked.open.../riv/klicoveSlovo
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  • NL - Nizozemsko
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  • [AEDFF0425264]
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  • Analytica Chimica Acta
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  • 812
http://linked.open...iv/tvurceVysledku
  • Šlais, Karel
  • Růžička, Filip
  • Kahle, Vladislav
  • Moravcová, Dana
  • Horká, Marie
  • Vykydalová, Marie
  • Duša, Filip
http://linked.open...ain/vavai/riv/wos
  • 000330581200033
issn
  • 0003-2670
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.aca.2013.12.032
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  • 14110
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