About: Lif1 SUMOylation and its role in non-homologous end-joining

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About: Lif1 SUMOylation and its role in non-homologous end-joining Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Non-homologous end-joining (NHEJ) repairs DNA double-strand breaks by tethering and ligating the two DNA ends. The mechanisms regulating NHEJ efficiency and interplay between its components are not fully understood. Here, we identify and characterize the SUMOylation of budding yeast Lif1 protein, which is required for the ligation step in NHEJ. We show that Lif1 SUMOylation occurs throughout the cell cycle and requires the Siz SUMO ligases. Single-strand DNA, but not double-strand DNA or the Lif1 binding partner Nej1, is inhibitory to Lif1 SUMOylation. We identify lysine 301 as the major conjugation site and demonstrate that its replacement with arginine completely abolishes Lif1 SUMOylation in vivo and in vitro. The lif1-K301R mutant cells exhibit increased levels of NHEJ repair compared with wild-type cells throughout the cell cycle. This is likely due to the inhibitory effect of Lif1 SUMOylation on both its self-association and newly observed single-strand DNA binding activity. Non-homologous end-joining (NHEJ) repairs DNA double-strand breaks by tethering and ligating the two DNA ends. The mechanisms regulating NHEJ efficiency and interplay between its components are not fully understood. Here, we identify and characterize the SUMOylation of budding yeast Lif1 protein, which is required for the ligation step in NHEJ. We show that Lif1 SUMOylation occurs throughout the cell cycle and requires the Siz SUMO ligases. Single-strand DNA, but not double-strand DNA or the Lif1 binding partner Nej1, is inhibitory to Lif1 SUMOylation. We identify lysine 301 as the major conjugation site and demonstrate that its replacement with arginine completely abolishes Lif1 SUMOylation in vivo and in vitro. The lif1-K301R mutant cells exhibit increased levels of NHEJ repair compared with wild-type cells throughout the cell cycle. This is likely due to the inhibitory effect of Lif1 SUMOylation on both its self-association and newly observed single-strand DNA binding activity. (en)
Title	Lif1 SUMOylation and its role in non-homologous end-joining Lif1 SUMOylation and its role in non-homologous end-joining (en)
skos:prefLabel	Lif1 SUMOylation and its role in non-homologous end-joining Lif1 SUMOylation and its role in non-homologous end-joining (en)
skos:notation	RIV/00216224:14110/13:00066405!RIV14-GA0-14110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.100/02/0123), P(GA13-26629S), P(GAP207/12/2323)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Nikulenkov, Fedor Vigašová, Dana Slezáková, Zuzana Krejčí, Lumír Altmannová, Veronika Kolesár, Peter
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	84928
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/13:00066405
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DOUBLE-STRAND BREAKS; DNA-LIGASE-IV; SACCHAROMYCES-CEREVISIAE; CRYSTAL-STRUCTURE; FUNCTIONAL INTERACTION; REPAIR PATHWAY; YEAST SEPTINS/; MATING-TYPE; PROTEIN; SUMO (en)
http://linked.open.../riv/klicoveSlovo	PROTEIN CRYSTAL-STRUCTURE SACCHAROMYCES-CEREVISIAE DOUBLE-STRAND BREAKS SUMO DNA-LIGASE-IV FUNCTIONAL INTERACTION MATING-TYPE REPAIR PATHWAY YEAST SEPTINS/
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[6C0216B60F53]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Endonuclease and translocase activity in type I restriction-modification complexes St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC) SUMO a stability genomu
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Krejčí, Lumír Altmannová, Veronika Kolesár, Peter Sarangi, Prabha Zhao, Xiaolan Anrather, Dorothea Chovanec, Miroslav Gith, Rainer Nikulenkov, Fedor Slezáková, Zuzana Vigašová, Dana Vlasakova, Danusa
http://linked.open...ain/vavai/riv/wos	000319806600024
issn	0305-1048
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/nar/gkt236
http://localhost/t...ganizacniJednotka	14110

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