About: Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells

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About: Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The inevitable accumulation of chromosomal abnormalities in human embryonic stem cells (hESCs) during in vitro expansion represents a considerable obstacle for cell replacement therapies. To determine the source of chromosomal abnormalities, we examined hESCs maintained in culture for over 55 months for defects in telomere maintenance and DNA repair. Although prolonged culture affected neither telomerase activity nor nonhomologous end joining, the efficiency of base excision repair (BER) was significantly decreased and correlated with reduced expression of apurinic/apyrimidinic endonuclease 1 (APE1), the major nuclease required for BER. Interestingly, the expression of other BER enzymes was unchanged. Addition of human recombinant APE1 protein to nuclear extracts from late passage hESCs increased BER efficiency to the level typical of early passage hESCs. The inevitable accumulation of chromosomal abnormalities in human embryonic stem cells (hESCs) during in vitro expansion represents a considerable obstacle for cell replacement therapies. To determine the source of chromosomal abnormalities, we examined hESCs maintained in culture for over 55 months for defects in telomere maintenance and DNA repair. Although prolonged culture affected neither telomerase activity nor nonhomologous end joining, the efficiency of base excision repair (BER) was significantly decreased and correlated with reduced expression of apurinic/apyrimidinic endonuclease 1 (APE1), the major nuclease required for BER. Interestingly, the expression of other BER enzymes was unchanged. Addition of human recombinant APE1 protein to nuclear extracts from late passage hESCs increased BER efficiency to the level typical of early passage hESCs. (en)
Title	Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells (en)
skos:prefLabel	Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells Decrease in Abundance of apurinic/apyrimidinic Endonuclease Causes Failure of Base Excision Repair in Culture-Adapted Human Embryonic Stem Cells (en)
skos:notation	RIV/00216224:14110/13:00065577!RIV14-MZ0-14110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GBP302/12/G157), P(NS10237)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hampl, Aleš Fojtík, Petr Matulka, Kamil Rotrekl, Vladimír Hejnová, Renata Eiselleová, Lívia Krutá, Miriama Bárta, Tomáš Fajkus, Jiří Dvořák, Petr Dobšáková, Zuzana Bálek, Lukáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	68112
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/13:00065577
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	APE1; base excision repair; human embryonic stem cells; culture adaptation; genome instability (en)
http://linked.open.../riv/klicoveSlovo	APE1 base excision repair genome instability culture adaptation human embryonic stem cells
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[5AF4ACD1F67F]
http://linked.open...i/riv/nazevZdroje	Stem Cells
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	12 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Disection of mechanism behind chromosomal aberation cumulation during long term cultivation of human embryonic stem cells. Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Hampl, Aleš Bárta, Tomáš Fajkus, Jiří Fojtík, Petr Bálek, Lukáš Matulka, Kamil Eiselleová, Lívia Hejnová, Renata Krutá, Miriama Kunická, Zuzana Rotrekl, Vladimír
http://linked.open...ain/vavai/riv/wos	000316624300008
issn	1066-5099
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/stem.1312
http://localhost/t...ganizacniJednotka	14110

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