About: Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy

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About: Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	As electron microscopy can reveal much smaller changes in plasma membrane integrity than optical methods, it appears to be a useful tool for evaluating semen quality or the damage inflicted on spermatozoa during preservation. By evaluating membrane integrity of the sperm head in 16 boars by transmission electron microscopy (TEM), it was found that the plasma membrane is very sensitive and tends to break in the acrosomal area but not in the rest of the sperm head. The earliest signs of injury to spermatozoa in the acrosomal part of the sperm head were observed in the plasma membrane that became swollen, broken or lost. The next damaged part of spermatozoa which is less sensitive than the plasma membrane is the acrosome and its external membrane. The freezing process caused a substantial increase in the degree of cell damage manifested as disturbed or missing plasma membrane, acrosomal reaction-like changes and unevenly distributed or lost acrosomal contents. The latter was not found in fresh semen. As electron microscopy can reveal much smaller changes in plasma membrane integrity than optical methods, it appears to be a useful tool for evaluating semen quality or the damage inflicted on spermatozoa during preservation. By evaluating membrane integrity of the sperm head in 16 boars by transmission electron microscopy (TEM), it was found that the plasma membrane is very sensitive and tends to break in the acrosomal area but not in the rest of the sperm head. The earliest signs of injury to spermatozoa in the acrosomal part of the sperm head were observed in the plasma membrane that became swollen, broken or lost. The next damaged part of spermatozoa which is less sensitive than the plasma membrane is the acrosome and its external membrane. The freezing process caused a substantial increase in the degree of cell damage manifested as disturbed or missing plasma membrane, acrosomal reaction-like changes and unevenly distributed or lost acrosomal contents. The latter was not found in fresh semen. (en)
Title	Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy (en)
skos:prefLabel	Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy (en)
skos:notation	RIV/00216224:14110/12:00068283!RIV14-MSM-14110___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(QI111A166), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	č. 2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Sedláčková, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	125302
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/12:00068283
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Boar; sperm; membrane; TEM; ultrastructure (en)
http://linked.open.../riv/klicoveSlovo	ultrastructure Boar TEM sperm membrane
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[A89740E60A79]
http://linked.open...i/riv/nazevZdroje	Research in pig breeding
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnological processes in reproduction and pig breeding as a tool of the economic growth and competitive advantage.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	roč. 6
http://linked.open...iv/tvurceVysledku	Hlavicová, J. Kopecká, V. Přinosilová, P. Sedláčková, Miroslava
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1802-7547
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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