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  • Purpose: Herpes stromal keratitis is a serious condition and the most frequent cause of unilateral blindness. The real-time PCR is an accurate and fast diagnostic method for an analysis of infectious agents causing keratitis and keratouveitis. The aim of the study was to assess the relationship between clinical symptoms, treatment efficacy monitoring and viral quantity in corneal swabs determined by quantitative real-time PCR method. The real-time PCR method was used as well for the detection of other viral eye pathogens. Methods: A total of 212 patients (136 men and 76 women) suspect of having herpes simplex virus (HSV) keratitis or keratouveitis were included in the study. The detection and quantitative analysis of the viral DNA were performed using the EliGene HSV1 RT kit, and the result was correlated with the clinical picture of the disease. The patients were routinely treated with acyclovir applied locally or, alternatively, in systemic administration.
  • Purpose: Herpes stromal keratitis is a serious condition and the most frequent cause of unilateral blindness. The real-time PCR is an accurate and fast diagnostic method for an analysis of infectious agents causing keratitis and keratouveitis. The aim of the study was to assess the relationship between clinical symptoms, treatment efficacy monitoring and viral quantity in corneal swabs determined by quantitative real-time PCR method. The real-time PCR method was used as well for the detection of other viral eye pathogens. Methods: A total of 212 patients (136 men and 76 women) suspect of having herpes simplex virus (HSV) keratitis or keratouveitis were included in the study. The detection and quantitative analysis of the viral DNA were performed using the EliGene HSV1 RT kit, and the result was correlated with the clinical picture of the disease. The patients were routinely treated with acyclovir applied locally or, alternatively, in systemic administration. (en)
Title
  • The treatment of HSV1 ocular infections using quantitative real-time PCR results
  • The treatment of HSV1 ocular infections using quantitative real-time PCR results (en)
skos:prefLabel
  • The treatment of HSV1 ocular infections using quantitative real-time PCR results
  • The treatment of HSV1 ocular infections using quantitative real-time PCR results (en)
skos:notation
  • RIV/00216224:14110/12:00061211!RIV13-MZ0-14110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(NR8507), S, V
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 175043
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/12:00061211
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA diagnostics; herpes simplex virus 1; HSV1; keratitis; real-time PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [5C8F57AD8274]
http://linked.open...i/riv/nazevZdroje
  • ACTA OPHTHALMOLOGICA
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 90
http://linked.open...iv/tvurceVysledku
  • Hlinomazová, Zuzana
  • Šerý, Omar
  • Horáčková, Monika
  • Loukotová, Věra
http://linked.open...ain/vavai/riv/wos
  • 000306903600035
issn
  • 1755-375X
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/j.1755-3768.2010.01933.x
http://localhost/t...ganizacniJednotka
  • 14110
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