About: HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS     Goto   Sponge   NotDistinct   Permalink

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  • The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion.
  • The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion. (en)
Title
  • HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
  • HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS (en)
skos:prefLabel
  • HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
  • HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS (en)
skos:notation
  • RIV/00216224:14110/10:00051723!RIV12-MSM-14110___
http://linked.open...avai/riv/aktivita
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  • I
http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 261506
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/10:00051723
http://linked.open...riv/jazykVysledku
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  • K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [92130CA74A75]
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  • Biomedical Papers
http://linked.open...in/vavai/riv/obor
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http://linked.open...v/svazekPeriodika
  • 154
http://linked.open...iv/tvurceVysledku
  • Šrámek, Vladimír
  • Hruda, Jan
  • Leverve, Xavier
http://linked.open...ain/vavai/riv/wos
  • 000285643800007
issn
  • 1213-8118
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  • 14110
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