About: Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy

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About: Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. During conidiogenesis,mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. Conclusion: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule. The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. During conidiogenesis,mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. Conclusion: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule. (en)
Title	Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy (en)
skos:prefLabel	Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy (en)
skos:notation	RIV/00216224:14110/10:00048691!RIV11-GA0-14110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA310/06/0605)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ramíková, Vladimíra Ilkovics, Ladislav Kopecká, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	255927
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/10:00048691
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capsule; Conidiogenesis; Latrunculin A; Methylbenzimidazole-2-ylcarbamate; Scanning electron microscopy; Yeast (en)
http://linked.open.../riv/klicoveSlovo	Capsule Latrunculin A Methylbenzimidazole-2-ylcarbamate Scanning electron microscopy Yeast Conidiogenesis
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[21AAB80556BB]
http://linked.open...i/riv/nazevZdroje	Chemotherapy
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Human pathogenic fungi : the cytoskeleton as a potential target of inhibition of morphogenesis.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Kopecká, Marie Yamaguchi, Masashi Ilkovics, Ladislav Ramíková, Vladimíra
http://linked.open...ain/vavai/riv/wos	00278957700005
issn	0009-3157
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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