About: Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy

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About: Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Receptor for Advanced Glycation End-products (RAGE) belongs to the family of pattern-recognition receptors and is significantly involved in the molecular mechanisms mediating pro-inflammatory action of hyperglycemia in diabetes. The aim of the current study was to elucidate the possible functional impact of the genetic variability in the AGER gene constituting previously identified risk haplotype for diabetic nephropathy (-429C/-374T/2184G) by testing the haplotype-specific effect on the AGER gene transcriptional activity in vitro. Promotor and intron 8 constructs carrying respective substitutions were amplified and cloned into pGL3-Basic reporter vector and subsequently used for transfection of HEK293 cells. Following 48hrs incubation in either normo- (5 mM/L) or hyperglycemic (25 mM/L) culture medium luciferase activity was measured to assess transcriptional efficiency. The Receptor for Advanced Glycation End-products (RAGE) belongs to the family of pattern-recognition receptors and is significantly involved in the molecular mechanisms mediating pro-inflammatory action of hyperglycemia in diabetes. The aim of the current study was to elucidate the possible functional impact of the genetic variability in the AGER gene constituting previously identified risk haplotype for diabetic nephropathy (-429C/-374T/2184G) by testing the haplotype-specific effect on the AGER gene transcriptional activity in vitro. Promotor and intron 8 constructs carrying respective substitutions were amplified and cloned into pGL3-Basic reporter vector and subsequently used for transfection of HEK293 cells. Following 48hrs incubation in either normo- (5 mM/L) or hyperglycemic (25 mM/L) culture medium luciferase activity was measured to assess transcriptional efficiency. (en)
Title	Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy (en)
skos:prefLabel	Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy (en)
skos:notation	RIV/00216224:14110/10:00040436!RIV11-MSM-14110___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(KJB501620601), S
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kuchtíčková, Šárka Beneš, Petr Kaňková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	259931
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/10:00040436
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	RAGE; hyperglycemia; transcriptional activity; haplotype; reporter assay (en)
http://linked.open.../riv/klicoveSlovo	haplotype hyperglycemia RAGE reporter assay transcriptional activity
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[76BA4BFA63FC]
http://linked.open...i/riv/nazevZdroje	Experimental and Clinical Endocrinology & Diabetes
http://linked.open...in/vavai/riv/obor	FB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional analysis of the RAGE gene susceptibility haplotype and its role in the hyperglycemia-driven pathology
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	118
http://linked.open...iv/tvurceVysledku	Beneš, Petr Kaňková, Kateřina Kuchtíčková, Šárka
issn	0947-7349
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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