About: Comparative study of mouse and human feeder cells for human embryonic stem cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Comparative study of mouse and human feeder cells for human embryonic stem cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. Here, we compared the expression and secretion of four well-established regulators of hESC pluripotency and/or differentiation among five lines of human foreskin fibroblasts and primary mouse embryonic fibroblasts throughout a standard hESC culture procedure. We found that production of TGFbeta1, activin A, and FGF-2 varied considerably among the human-derived feeder cell lines. Low- and high-producing human feeder cells as well as mouse feeder cells were evaluated for their ability to support the undifferentiated growth of hESCs and for the expression of stem cell markers. Our results suggest that the ability of a feeder layer to promote the undifferentiated growth of hESCs is attributable to its characteristic growth factor production. Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. Here, we compared the expression and secretion of four well-established regulators of hESC pluripotency and/or differentiation among five lines of human foreskin fibroblasts and primary mouse embryonic fibroblasts throughout a standard hESC culture procedure. We found that production of TGFbeta1, activin A, and FGF-2 varied considerably among the human-derived feeder cell lines. Low- and high-producing human feeder cells as well as mouse feeder cells were evaluated for their ability to support the undifferentiated growth of hESCs and for the expression of stem cell markers. Our results suggest that the ability of a feeder layer to promote the undifferentiated growth of hESCs is attributable to its characteristic growth factor production. (en)
Title	Comparative study of mouse and human feeder cells for human embryonic stem cells Comparative study of mouse and human feeder cells for human embryonic stem cells (en)
skos:prefLabel	Comparative study of mouse and human feeder cells for human embryonic stem cells Comparative study of mouse and human feeder cells for human embryonic stem cells (en)
skos:notation	RIV/00216224:14110/08:00026133!RIV10-GA0-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0538), P(GA305/05/0434), P(LC06077), Z(AV0Z50390512), Z(AV0Z50390703), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	52
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Neradil, Jakub Hampl, Aleš Slaninová, Iva Eiselleová, Lívia Peterková, Iveta Dvořák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	360514
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/08:00026133
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	hESCs; human embryonic stem cell; growth factor production; undifferentiated growth (en)
http://linked.open.../riv/klicoveSlovo	growth factor production hESCs human embryonic stem cell undifferentiated growth
http://linked.open...odStatuVydavatele	ES - Španělské království
http://linked.open...ontrolniKodProRIV	[097F90D3F883]
http://linked.open...i/riv/nazevZdroje	The International journal of developmental biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Fibroblast growth factor signaling pathways in human embryonic stem cells Center of Chemical Genetics
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Hampl, Aleš Neradil, Jakub Slaninová, Iva Eiselleová, Lívia Peterková, Iveta
http://linked.open...ain/vavai/riv/wos	000256177000004
http://linked.open...n/vavai/riv/zamer	Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie New biotechnologies, nanomaterials and stem cells for use in regenerative medicine Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0214-6282
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 76 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software