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Description
  • The isoelectric points of many microbial cells lie within the pH range spanning from 1.5 to 4.5. In this work, we suggest a CIEF method for the separation of cells according to their isoelectric points in the pH range of 2-5. It includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). This polymer dissolved in the catholyte, in the anolyte and in the injected sample pulse was used for a prevention of the bioanalyte adsorption on the capillary surface and for the reduction of the electroosmotic flow. Between each focusing run, the capillaries were washed with the mixture of acetone/ethanol to achieve the reproducible and efficient CIEF. In order to trace of pH gradients, low-molecular-mass pI markers were used. The mixed cultures of microorganisms, Escherichia coli CCM 3954, Candida albicans CCM 8180, Ca (en)
  • CIEF v kyselém gradientu pH 2-5 umožňuje separaci a identifikaci mikrobů i ze směsných kultur.
  • CIEF v kyselém gradientu pH 2-5 umožňuje separaci a identifikaci mikrobů i ze směsných kultur. (cs)
Title
  • Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí
  • Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. (en)
  • Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí (cs)
skos:prefLabel
  • Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí
  • Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. (en)
  • Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí (cs)
skos:notation
  • RIV/00216224:14110/06:00016448!RIV08-GA0-14110___
http://linked.open.../vavai/riv/strany
  • 840-846
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA301/99/0045)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 481423
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/06:00016448
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • capillary isoelectric focusing; microorganisms; Staphylococcus; Candida; Escherichia coli; UV detection. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [EFD5FFCF8844]
http://linked.open...i/riv/nazevZdroje
  • Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 385
http://linked.open...iv/tvurceVysledku
  • Holá, Veronika
  • Šlais, Karel
  • Růžička, Filip
  • Horká, Marie
issn
  • 1618-2642
number of pages
http://localhost/t...ganizacniJednotka
  • 14110
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