About: Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid

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About: Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This study employs mouse embryonal carcinoma (EC) cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. The data show that mouse EC cells are able to produce neural cells upon exposure to RA even without culture in three-dimensional embryoid bodies (EBs). However, in contrast to standard EBs-involving protocol(s), neural differentiation in monolayer only takes place when complex signaling from serum factors is avoided. This simple and efficient strategy is proposed to serve as a basis for neurodifferentiation studies in vitro. This study employs mouse embryonal carcinoma (EC) cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. The data show that mouse EC cells are able to produce neural cells upon exposure to RA even without culture in three-dimensional embryoid bodies (EBs). However, in contrast to standard EBs-involving protocol(s), neural differentiation in monolayer only takes place when complex signaling from serum factors is avoided. This simple and efficient strategy is proposed to serve as a basis for neurodifferentiation studies in vitro. (en)
Title	Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum (en)
skos:prefLabel	Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum (en)
skos:notation	RIV/00216224:14110/05:00021439!RIV10-GA0-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP524/03/P171), P(LN00A065), Z(AV0Z5039906), Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hampl, Aleš Bryja, Vítězslav Dvořák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	532542
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/05:00021439
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cells (en)
http://linked.open.../riv/klicoveSlovo	cells
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[31CCA8EDF29A]
http://linked.open...i/riv/nazevZdroje	Physiol. Res.
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Mechanisms driving neural differentiation in mouse embryonic stem cells in vitro
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Bryja, Vítězslav Dvořák, Petr Hampl, Aleš Pacherník, J. Kubala, L. Ešner, M.
http://linked.open...ain/vavai/riv/wos	000227607100014
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20432100001 The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy
issn	0862-8408
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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