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Description
  • Anotace česky: Byl vyšetřen transkriptom Treponema pallidum během experimentální infekce králíků pomocí DNA microarray čipů obsahujících všech 1039 anotovaných ORF. Geny Treponema pallidum byly rozděleny do skupin podle úrovně transkripce. (cs)
  • DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the tr
  • DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the tr (en)
Title
  • Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků (cs)
  • Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection
  • Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection (en)
skos:prefLabel
  • Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků (cs)
  • Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection
  • Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection (en)
skos:notation
  • RIV/00216224:14110/03:00012310!RIV/2005/MZ0/141105/N
http://linked.open.../vavai/riv/strany
  • 311
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(NI7351)
http://linked.open...iv/cisloPeriodika
  • 97
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 631287
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/03:00012310
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • transcriptome, Treponema pallidum, gene expression profiling (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [248FFAD8563A]
http://linked.open...i/riv/nazevZdroje
  • CHEMICKÉ LISTY
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2003
http://linked.open...iv/tvurceVysledku
  • Matějková, Petra
  • Šmajs, David
  • Norris, S. J.
  • Weinstock, G. M.
issn
  • 0009-2770
number of pages
http://localhost/t...ganizacniJednotka
  • 14110
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