| Attributes | Values |
|---|
| rdf:type
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| Description
| - Sensitivity of spheroplasts (prepared in two ways) of a colicin-sensitive strain, of colicin-resistant and of colicin-tolerant mutants and of strains immune to colicins E1 and E2 was estimated and compared. Generally, the removal of the peptidoglycan layer brought about a slight nonspecific support for colicin translocation across the cell wall in sensitive, tolB tolerant and immune bacteria. tolB spheroplasts were colicin E1-sensitive, but E2-insensitive. Spheroplasts were always fragile and lysed spontaneously, especially those produced by lysozyme. Bacteria carrying tolA, tolQ and tolR mutations kept their colicin insensitivity as spheroplasts, just as the resistant ones. Bacteria rendered colicinogenic and hence colicin-immune turned to high colicin sensitivity in spheroplast form. the results indicate a change in plasma membrane associated with the spheroplast formation.
- Sensitivity of spheroplasts (prepared in two ways) of a colicin-sensitive strain, of colicin-resistant and of colicin-tolerant mutants and of strains immune to colicins E1 and E2 was estimated and compared. Generally, the removal of the peptidoglycan layer brought about a slight nonspecific support for colicin translocation across the cell wall in sensitive, tolB tolerant and immune bacteria. tolB spheroplasts were colicin E1-sensitive, but E2-insensitive. Spheroplasts were always fragile and lysed spontaneously, especially those produced by lysozyme. Bacteria carrying tolA, tolQ and tolR mutations kept their colicin insensitivity as spheroplasts, just as the resistant ones. Bacteria rendered colicinogenic and hence colicin-immune turned to high colicin sensitivity in spheroplast form. the results indicate a change in plasma membrane associated with the spheroplast formation. (en)
- Byly zjištěna a porovnána senzitivita sféroplastů (připravených dvojím způsobem) kmene sensitivního, rezistentního, tolerantního a imunního ke kolicinům E1 a E2. Obecně platí, že odstranění peptidoglykanové vrstvy přineslo nespecifický promoční efekt na translokaci kolicinu přes buněčnou stěnu u senzitivního, tolB tolerantního a imunního kmene. Sféropolasty tolB tolerantního kmene byly citlivé ke kolicinu E1 ale necitlivé ke kolicinu E2. Sféroplasty byly fragilní a spontánně lyzovaly, zvláště ty připravené působením lysozymu. Bakterie nesoucí mutace v genech tolA, tolQ a tolR byly necitlivé ke kolicinům také ve formě sféroplastů, stejně jako bakterie rezistentní. Bakterie kolicinogenní a tedy imunní ke kolicinům se po přeměně ve sféroplasty staly senzitivní ke kolicinu. Výsledky naznačují změny ve struktuře a funkci plazmatické membrány během změny tyčinek ve sféroplasty. (cs)
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| Title
| - Traslokace kolicinu od receptoru k plazmatické membráně: funkce peptidoglykanové vrstvy (cs)
- Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer.
- Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer. (en)
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| skos:prefLabel
| - Traslokace kolicinu od receptoru k plazmatické membráně: funkce peptidoglykanové vrstvy (cs)
- Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer.
- Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer. (en)
|
| skos:notation
| - RIV/00216224:14110/02:00024520!RIV09-GA0-14110___
|
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14110/02:00024520
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - colicin; peptidoglycan layer (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Matějková, Petra
- Šmarda, Jan
- Vavříčková, Anna
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
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