| Attributes | Values |
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| rdf:type
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| Description
| - Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of
- Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of (en)
- Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of (cs)
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| Title
| - Detection of spectrin-like protein in yeasts: spectrin-actin relations
- Detection of spectrin-like protein in yeasts: spectrin-actin relations (en)
- Detection of spectrin-like protein in yeasts: spectrin-actin relations (cs)
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| skos:prefLabel
| - Detection of spectrin-like protein in yeasts: spectrin-actin relations
- Detection of spectrin-like protein in yeasts: spectrin-actin relations (en)
- Detection of spectrin-like protein in yeasts: spectrin-actin relations (cs)
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| skos:notation
| - RIV/00216224:14110/02:00006732!RIV08-GA0-14110___
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA204/00/0394), P(GA204/99/D025)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14110/02:00006732
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - yeast; spectrin; cytoskeleton; actin (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Sborník X. Cytoskeletálního klubu
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Slaninová, Iva
- Svoboda, Augustin
- Holubářová, Alena
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Československá biologická společnost
|
| http://localhost/t...ganizacniJednotka
| |
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