About: Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae

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About: Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise. The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise. (en)
Title	Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae (en)
skos:prefLabel	Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae (en)
skos:notation	RIV/00216224:14110/01:00005112!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany	325
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/00/0394), P(GA204/99/D025)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Slaninová, Iva Holubářová, Alena Svoboda, Augustin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	676755
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/01:00005112
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	yeast, protoplasts, cell wall, actin, microtubules, cell polarity (en)
http://linked.open.../riv/klicoveSlovo	actin yeast cell wall protoplasts microtubules cell polarity
http://linked.open...odStatuVydavatele	HU - Maďarsko
http://linked.open...ontrolniKodProRIV	[95882DBA72C6]
http://linked.open...i/riv/nazevZdroje	Acta Biologica Hungarica
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions The role of cytoskeleton and cell wall in the cell polarization
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Slaninová, Iva Svoboda, Augustin Holubářová, Alena
issn	0236-5383
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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