About: Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts     Goto   Sponge   NotDistinct   Permalink

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  • Actin cytoskeleton and microtubules were studied in a human fungal pathogen, the basidiomycetous yeast Cryptococcus neoformans (haploid phase of Filobasidiella neoformans), during its asexual reproduction by budding using fluorescence and electron microscopy. Staining with rhodamine-conjugated phalloidin revealed an F-actin cytoskeleton consisting of cortical patches, cables and cytokinetic ring. F-actin patches accumulated at the regions of cell wall growth, i.e. in cylindrical sterigma-like protrusion, bud and septum. In mother cells evenly distributed F-actin patches were joined to F-actin cables, which were directed to the growing sterigma-like protrusion and bud. Some F-actin cables were associated with the cell nucleus. The F-actin cytokinetic ring was located in the bud neck, where the septum originated. Anti-tubulin TAT1 antibody revealed a microtubular cytoskeleton consisting of cytoplasmic and spindle microtubules. In interphase cells cytoplasmic microtubules pointed to the growin
  • Actin cytoskeleton and microtubules were studied in a human fungal pathogen, the basidiomycetous yeast Cryptococcus neoformans (haploid phase of Filobasidiella neoformans), during its asexual reproduction by budding using fluorescence and electron microscopy. Staining with rhodamine-conjugated phalloidin revealed an F-actin cytoskeleton consisting of cortical patches, cables and cytokinetic ring. F-actin patches accumulated at the regions of cell wall growth, i.e. in cylindrical sterigma-like protrusion, bud and septum. In mother cells evenly distributed F-actin patches were joined to F-actin cables, which were directed to the growing sterigma-like protrusion and bud. Some F-actin cables were associated with the cell nucleus. The F-actin cytokinetic ring was located in the bud neck, where the septum originated. Anti-tubulin TAT1 antibody revealed a microtubular cytoskeleton consisting of cytoplasmic and spindle microtubules. In interphase cells cytoplasmic microtubules pointed to the growin (en)
Title
  • Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts
  • Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts (en)
skos:prefLabel
  • Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts
  • Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts (en)
skos:notation
  • RIV/00216224:14110/01:00003574!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany
  • 303-311
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA310/00/0391)
http://linked.open...iv/cisloPeriodika
  • April issu
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 686936
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/01:00003574
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cryptococcus neoformans-cytoskeleton-actin-microtubules-Saccharomyces-Schizosaccharomyces (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [B57344D2B88A]
http://linked.open...i/riv/nazevZdroje
  • Europen Journal of Cell Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 80
http://linked.open...iv/tvurceVysledku
  • Kopecká, Marie
  • Yamaguchi, Masashi
  • Svoboda, Augustin
  • Gabriel, Miroslav
  • Ohkusu, Misako
  • Takeo, Kanji
  • Hata, Kunihiko
  • Yoshida, Soichi
issn
  • 0171-9335
number of pages
http://localhost/t...ganizacniJednotka
  • 14110
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