About: Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.chemphyslip.2013.04.002
Description	Drop coating deposition Raman (DCDR) spectroscopy was used to study liposomes (DPPC and asolectin) with growing proportion of cholesterol. Deposited samples of both liposomes on special hydrophobic surface formed a dried drop with a circular shape with a ring of concentrated liposomes at the outer edge. The presence of cholesterol in liposome causes a diminishing of the drop size and an increasing in diameter of the ring, but DPPC with 20% of cholesterol forms the compact drop without the ring. Raman spectra contain characteristics of both lipids and cholesterol, liposomes do not change their initial phase state after drying. Spectral mapping shows that maximum Raman intensity originated from the inner part of the ring. Our results suggest that DCDR spectroscopy can be used for studying lipids containing cholesterol in situ. Drop coating deposition Raman (DCDR) spectroscopy was used to study liposomes (DPPC and asolectin) with growing proportion of cholesterol. Deposited samples of both liposomes on special hydrophobic surface formed a dried drop with a circular shape with a ring of concentrated liposomes at the outer edge. The presence of cholesterol in liposome causes a diminishing of the drop size and an increasing in diameter of the ring, but DPPC with 20% of cholesterol forms the compact drop without the ring. Raman spectra contain characteristics of both lipids and cholesterol, liposomes do not change their initial phase state after drying. Spectral mapping shows that maximum Raman intensity originated from the inner part of the ring. Our results suggest that DCDR spectroscopy can be used for studying lipids containing cholesterol in situ. (en)
Title	Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol (en)
skos:prefLabel	Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol (en)
skos:notation	RIV/00216208:11320/13:10189236!RIV14-GA0-11320___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP208/10/0376)
http://linked.open...iv/cisloPeriodika	Neuveden
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kočišová, Eva Procházka, Marek Antalík, Andrej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	70602
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/13:10189236
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cholesterol; DPPC; Asolectin; Liposome; Drop coating deposition Raman spectroscopy (en)
http://linked.open.../riv/klicoveSlovo	Asolectin DPPC Drop coating deposition Raman spectroscopy Cholesterol Liposome
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A07B58FB7EC9]
http://linked.open...i/riv/nazevZdroje	Chemistry and Physics of Lipids
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Interaction of antibacterial peptides with the model membranes and possibility of the prediction of their biological activity
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	172
http://linked.open...iv/tvurceVysledku	Kočišová, Eva Procházka, Marek Antalík, Andrej
http://linked.open...ain/vavai/riv/wos	000322415100001
issn	0009-3084
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chemphyslip.2013.04.002
http://localhost/t...ganizacniJednotka	11320

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