About: Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo     Goto   Sponge   NotDistinct   Permalink

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Description
  • Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from 0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines.
  • Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from 0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines. (en)
Title
  • Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo
  • Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo (en)
skos:prefLabel
  • Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo
  • Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo (en)
skos:notation
  • RIV/00216208:11320/13:10173450!RIV14-MSM-11320___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika
  • Nov 2013
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 106975
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11320/13:10173450
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cells; PDT; Singlet oxygen; Protoporphyrin IX; TMPyP; TPPS4 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [54DF58D1A52D]
http://linked.open...i/riv/nazevZdroje
  • Journal of Luminescence
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 143
http://linked.open...iv/tvurceVysledku
  • Vyklický, Vojtěch
  • Dědic, Roman
  • Hála, Jan
  • Curkaniuk, Natalija
http://linked.open...ain/vavai/riv/wos
  • 000323912400119
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-2313
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.jlumin.2011.12.033
http://localhost/t...ganizacniJednotka
  • 11320
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