About: Spectroscopic Determination of pKa Constants of MADS Box Segments

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1155/2012/674032
Description	We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states. The method is based on the factor analysis of multiwavelength spectroscopic pH titration data. As an illustration, we present its application on the study of short segments of the MADS box, which is a highly conserved sequence of a so-called family of transcription factors, by techniques of UV absorption and fluorescence spectroscopies. Investigated oligopeptides contain no tryptophan but one tyrosine serving as an intrinsic fluorophore and absorber. The results indicate both good sensitivity and spectroscopic selectivity of our method, which thus may be considered as a complementary technique to conventional electrochemical methods. We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states. The method is based on the factor analysis of multiwavelength spectroscopic pH titration data. As an illustration, we present its application on the study of short segments of the MADS box, which is a highly conserved sequence of a so-called family of transcription factors, by techniques of UV absorption and fluorescence spectroscopies. Investigated oligopeptides contain no tryptophan but one tyrosine serving as an intrinsic fluorophore and absorber. The results indicate both good sensitivity and spectroscopic selectivity of our method, which thus may be considered as a complementary technique to conventional electrochemical methods. (en)
Title	Spectroscopic Determination of pKa Constants of MADS Box Segments Spectroscopic Determination of pKa Constants of MADS Box Segments (en)
skos:prefLabel	Spectroscopic Determination of pKa Constants of MADS Box Segments Spectroscopic Determination of pKa Constants of MADS Box Segments (en)
skos:notation	RIV/00216208:11320/12:10129610!RIV13-GA0-11320___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GA202/09/0193), S
http://linked.open...iv/cisloPeriodika	5-6
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Štěpánek, Josef Profantová, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	170371
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/12:10129610
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	tyrosine; MADS box; fluorescence spectroscopy; dissociation constant; Factor analysis (en)
http://linked.open.../riv/klicoveSlovo	fluorescence spectroscopy dissociation constant Factor analysis MADS box tyrosine
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C1E7729514E5]
http://linked.open...i/riv/nazevZdroje	SPECTROSCOPY-AN INTERNATIONAL JOURNAL
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Formation and dynamics of nucleic acid motifs involved in regulation of gene expression
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Turpin, Pierre-Yves Štěpánek, Josef Coic, Yves-Marie Profantová, Barbora Zentz, Christian
http://linked.open...ain/vavai/riv/wos	000306505500026
issn	0712-4813
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1155/2012/674032
http://localhost/t...ganizacniJednotka	11320

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