About: SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1155/2012/393847
Description	We report the application of dried Ag hydroxylamine-reduced colloidal drops to surface-enhanced (resonance) Raman scattering (SE(R)RS) study of biomolecules using Raman microspectroscopy. 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (TMPyP), amino acid tryptophan, and phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) served as testing biomolecules. Ag colloid/biomolecule drop dried on glass support forms a ring in the edge part of the drop in which almost all nanoparticles are clustered. This specific drying process promotes adsorption of the studied biomolecules in highly enhancing sites (%22hot spots%22) as well as concentrates them in the ring. We were able to obtain SE(R) RS spectra from the ring that cannot be acquired directly from Ag colloidal solutions (SERRS spectrum of 1 x 10(-10) M TMPyP by 1 s accumulation time, SERS spectrum of 2 x 10(-7) M DSPC). Despite the spectral irreproducibility and problems with spurious bands in some cases, SERS microspectroscopy of studied biomolecules using dried Ag colloid/adsorbate systems improves SERS applicability and sensitivity in comparison to measurements directly from Ag colloidal solution. We report the application of dried Ag hydroxylamine-reduced colloidal drops to surface-enhanced (resonance) Raman scattering (SE(R)RS) study of biomolecules using Raman microspectroscopy. 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (TMPyP), amino acid tryptophan, and phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) served as testing biomolecules. Ag colloid/biomolecule drop dried on glass support forms a ring in the edge part of the drop in which almost all nanoparticles are clustered. This specific drying process promotes adsorption of the studied biomolecules in highly enhancing sites (%22hot spots%22) as well as concentrates them in the ring. We were able to obtain SE(R) RS spectra from the ring that cannot be acquired directly from Ag colloidal solutions (SERRS spectrum of 1 x 10(-10) M TMPyP by 1 s accumulation time, SERS spectrum of 2 x 10(-7) M DSPC). Despite the spectral irreproducibility and problems with spurious bands in some cases, SERS microspectroscopy of studied biomolecules using dried Ag colloid/adsorbate systems improves SERS applicability and sensitivity in comparison to measurements directly from Ag colloidal solution. (en)
Title	SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops (en)
skos:prefLabel	SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops (en)
skos:notation	RIV/00216208:11320/12:10126624!RIV13-GA0-11320___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GAP208/10/0941), S
http://linked.open...iv/cisloPeriodika	5-6
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kočišová, Eva Procházka, Marek Šimáková, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	167553
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/12:10126624
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phospolipid; tryptophan; porphyrins; microspectroscopy; SERS (en)
http://linked.open.../riv/klicoveSlovo	porphyrins SERS microspectroscopy phospolipid tryptophan
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[48B838650EED]
http://linked.open...i/riv/nazevZdroje	SPECTROSCOPY-AN INTERNATIONAL JOURNAL
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Engineering of surface-modified optical processes in molecules and semiconductor quantum dots by plasmon resonances in metal nanoparticle assemblies.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Kočišová, Eva Procházka, Marek Šimáková, Petra
http://linked.open...ain/vavai/riv/wos	000306505500025
issn	0712-4813
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1155/2012/393847
http://localhost/t...ganizacniJednotka	11320

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