About: Maximum Entropy Analysis of Analytically Simulated Complex Fluorescence Decays

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.springerlink.com/content/9578477601965430/fulltext.pdf
Description	We tested a Maximum Entropy Method developed for oversampled data (SVD-MEM) on complex analytically simulated exponential decay data consisting of both noisy and noiseless multi-exponential fluorescence decay curves. We observed recovery of simulated parameters for three sets of data: a decay containing three exponential functions in both intensity and anisotropy curves, a set of intensity decays composed of 4, 5 and 6 exponential functions, and a decay characterized by a Gaussian lifetime distribution. The SVD-MEM fitting of the noiseless data returned the simulated parameters with the high accuracy. Noise added to the data affected recovery of the parameters in dependence on a data complexity. At selected realistic noise levels we obtained a good recovery of simulated parameters for all tested data sets. Decay parameters recovered from decays containing discrete lifetime components were almost independent of the value of the entropy scaling parameter gamma used in the maximization procedure when it changed across the main peak of its posterior probability. A correct recovery of the Gaussian shaped lifetime distribution required selection of the gamma-factor which was by several orders of magnitude larger than its most probable value to avoid a band splitting. We tested a Maximum Entropy Method developed for oversampled data (SVD-MEM) on complex analytically simulated exponential decay data consisting of both noisy and noiseless multi-exponential fluorescence decay curves. We observed recovery of simulated parameters for three sets of data: a decay containing three exponential functions in both intensity and anisotropy curves, a set of intensity decays composed of 4, 5 and 6 exponential functions, and a decay characterized by a Gaussian lifetime distribution. The SVD-MEM fitting of the noiseless data returned the simulated parameters with the high accuracy. Noise added to the data affected recovery of the parameters in dependence on a data complexity. At selected realistic noise levels we obtained a good recovery of simulated parameters for all tested data sets. Decay parameters recovered from decays containing discrete lifetime components were almost independent of the value of the entropy scaling parameter gamma used in the maximization procedure when it changed across the main peak of its posterior probability. A correct recovery of the Gaussian shaped lifetime distribution required selection of the gamma-factor which was by several orders of magnitude larger than its most probable value to avoid a band splitting. (en)
Title	Maximum Entropy Analysis of Analytically Simulated Complex Fluorescence Decays Maximum Entropy Analysis of Analytically Simulated Complex Fluorescence Decays (en)
skos:prefLabel	Maximum Entropy Analysis of Analytically Simulated Complex Fluorescence Decays Maximum Entropy Analysis of Analytically Simulated Complex Fluorescence Decays (en)
skos:notation	RIV/00216208:11320/11:10105611!RIV12-MSM-11320___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Heřman, Petr Večeř, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	210980
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/11:10105611
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Fluorescence decays; Analytical simulations; Maximum entropy method (en)
http://linked.open.../riv/klicoveSlovo	Analytical simulations Fluorescence decays Maximum entropy method
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[4E3B321A4EAA]
http://linked.open...i/riv/nazevZdroje	Journal of Fluorescence
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Heřman, Petr Večeř, Jaroslav
http://linked.open...ain/vavai/riv/wos	000291999400006
http://linked.open...n/vavai/riv/zamer	Fyzika molekulárních, makromolekulárních a biologických systémů
issn	1053-0509
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10895-009-0589-1
http://localhost/t...ganizacniJednotka	11320

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