About: Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls     Goto   Sponge   NotDistinct   Permalink

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  • Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
  • Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall. (en)
Title
  • Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls
  • Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls (en)
skos:prefLabel
  • Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls
  • Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls (en)
skos:notation
  • RIV/00216208:11320/10:10082434!RIV11-MSM-11320___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 288542
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11320/10:10082434
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cell wall; Yeast; Fluorescence; Calcofluor; Solvatochromic effect (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [9A3BF48236C5]
http://linked.open...i/riv/nazevZdroje
  • Journal of Fluorescence
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2010/20
http://linked.open...iv/tvurceVysledku
  • Plášek, Jaromír
  • Hošková, Barbora
http://linked.open...ain/vavai/riv/wos
  • 000274401600040
http://linked.open...n/vavai/riv/zamer
issn
  • 1053-0509
number of pages
http://localhost/t...ganizacniJednotka
  • 11320
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