About: Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Raman titration experiment with magnesium salt added gradually to aqueous solution of duplexes formed by RNA homopolynucleotides polyU and polyA was performed to reveal its effect on homopolynucleotide complexes. Statistical analysis of obtained spectral set has confirmed the effect already found by less structurally sensitive methods [Nucleic Acids Res. 31(17) (2003), 5101-5107] that at sufficiently high concentrations magnesium causes transformation of polyU-polyA duplexes to polyU-polyA-polyU triplexes and single polyA strands. It was found that at relatively high polynucleotide concentrations used in Raman experiment, the threshold magnesium concentration for this effect is above the concentration of duplex base-pairs in solution. Due to the strong spectral changes attributed to the varied percentages of duplexes, triplexes and single strands, it was not possible to register weaker direct Raman signs of the magnesium binding to polynucleotide strand. Raman titration experiment with magnesium salt added gradually to aqueous solution of duplexes formed by RNA homopolynucleotides polyU and polyA was performed to reveal its effect on homopolynucleotide complexes. Statistical analysis of obtained spectral set has confirmed the effect already found by less structurally sensitive methods [Nucleic Acids Res. 31(17) (2003), 5101-5107] that at sufficiently high concentrations magnesium causes transformation of polyU-polyA duplexes to polyU-polyA-polyU triplexes and single polyA strands. It was found that at relatively high polynucleotide concentrations used in Raman experiment, the threshold magnesium concentration for this effect is above the concentration of duplex base-pairs in solution. Due to the strong spectral changes attributed to the varied percentages of duplexes, triplexes and single strands, it was not possible to register weaker direct Raman signs of the magnesium binding to polynucleotide strand. (en)
Title	Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes (en)
skos:prefLabel	Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes (en)
skos:notation	RIV/00216208:11320/10:10072373!RIV11-GA0-11320___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA202/09/0193), P(GD202/09/H041), S, Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Štěpánek, Josef Espinoza Herrera, Shirly Josefina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	283855
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/10:10072373
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	polyA; polyU; triplex; duplex; magnesium; Raman (en)
http://linked.open.../riv/klicoveSlovo	polyA Raman triplex duplex polyU magnesium
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7B5E5948850E]
http://linked.open...i/riv/nazevZdroje	SPECTROSCOPY-AN INTERNATIONAL JOURNAL
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Physics of nanostructures Formation and dynamics of nucleic acid motifs involved in regulation of gene expression
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Štěpánek, Josef Espinoza Herrera, Shirly Josefina
http://linked.open...ain/vavai/riv/wos	000281667400041
http://linked.open...n/vavai/riv/zamer	Fyzika molekulárních, makromolekulárních a biologických systémů
issn	0712-4813
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	11320

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