About: Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study

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About: Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The energetic landscape of the allosteric regulatory mechanism of RMPK was characterized by isothermal titration calorimetry (ITC). ITC data for RMPK binding to substrates PEP and ADP, for the allosteric inhibitor Phe, and for combination of ADP and Phe were globally analyzed. The thermodynamic parameters characterizing the linked-multiple-equilibrium system were extracted. Four novel insights were uncovered. (1) The binding preference of ADP for either the T or R state is temperature-dependent, namely, more favorable to the T and R states at high and low temperatures, respectively. Depending on the temperature, binding of ADP can regulate RMPK activity by favoring the enzyme to either the R or T state. (2) The binding of Phe is negatively coupled to that of ADP (3) The release or absorption of protons linked to the various equilibria is specific to the particular reaction. (4) The R {--} T equilibrium is accompanied by a significant DeltaC(p). The energetic landscape of the allosteric regulatory mechanism of RMPK was characterized by isothermal titration calorimetry (ITC). ITC data for RMPK binding to substrates PEP and ADP, for the allosteric inhibitor Phe, and for combination of ADP and Phe were globally analyzed. The thermodynamic parameters characterizing the linked-multiple-equilibrium system were extracted. Four novel insights were uncovered. (1) The binding preference of ADP for either the T or R state is temperature-dependent, namely, more favorable to the T and R states at high and low temperatures, respectively. Depending on the temperature, binding of ADP can regulate RMPK activity by favoring the enzyme to either the R or T state. (2) The binding of Phe is negatively coupled to that of ADP (3) The release or absorption of protons linked to the various equilibria is specific to the particular reaction. (4) The R {--} T equilibrium is accompanied by a significant DeltaC(p). (en)
Title	Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study (en)
skos:prefLabel	Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 1. Calorimetric study (en)
skos:notation	RIV/00216208:11320/09:00206213!RIV10-MSM-11320___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	40
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Heřman, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	315708
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/09:00206213
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Functional; energetic; landscape; allosteric; regulation; muscle; pyruvate; kinase; Calorimetric; study (en)
http://linked.open.../riv/klicoveSlovo	allosteric Calorimetric energetic pyruvate Functional landscape - the utilization of landscape - the Liechtenstein estate - south Moravia - Pozořice regulation study muscle kinase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B3BB68E70312]
http://linked.open...i/riv/nazevZdroje	Biochemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Heřman, Petr Lee, James C.
http://linked.open...ain/vavai/riv/wos	000270459100015
http://linked.open...n/vavai/riv/zamer	Fyzika molekulárních, makromolekulárních a biologických systémů
issn	0006-2960
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	11320

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