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| Description
| - Antisense strategy represents a molecular tool for selective chemotherapeutic action. It belongs among oligonucleotide strategies that employ specific single-stranded sequences of deoxy- and ribonucleotides or their synthetic analogs to block or suppress expression of a pathogen. However, the use of modified oligonucleotides is complicated by non-ideal properties of currently available analogs. Successful medical treatment requires proper binding of the modified oligonucleotide to its cellular target and also its efficient cellular uptake, stability and appropriate distribution in the intracellular environment. The latter processes can be effectively studied by microfluorescence techniques. The paper reviews the current situation in the application of advanced microfluorescence methods and gives a description of the oligonucleotide strategy and possibilities to support the cellular uptake,basics of current fluorescence microimaging and microspectroscopy including time-resolved measurements.
- Antisense strategy represents a molecular tool for selective chemotherapeutic action. It belongs among oligonucleotide strategies that employ specific single-stranded sequences of deoxy- and ribonucleotides or their synthetic analogs to block or suppress expression of a pathogen. However, the use of modified oligonucleotides is complicated by non-ideal properties of currently available analogs. Successful medical treatment requires proper binding of the modified oligonucleotide to its cellular target and also its efficient cellular uptake, stability and appropriate distribution in the intracellular environment. The latter processes can be effectively studied by microfluorescence techniques. The paper reviews the current situation in the application of advanced microfluorescence methods and gives a description of the oligonucleotide strategy and possibilities to support the cellular uptake,basics of current fluorescence microimaging and microspectroscopy including time-resolved measurements. (en)
- Antisensní strategie představuje nadějný molekulární nástroj pro efektivní a selektivní chemoterapeutické působení. Patří mezi oligonukleotidové strategie,které využívají specifické jednovláknové sekvence deoxyribo- a ribonukleotidů, nebo jejich syntetických analog k blokování nebo potlačení exprese pathogenu.Nicméně rutinní využití modifikovaných oligonukleotidů je komplikované vzhledem k neideálním vlastnostem dostupných analog. Úspěšné léčení vyžaduje nejen správnou vazbu modifikovaného oligonukleotidu na buněčný cíl, ale také efektivní transport do buňky, stabilitu a vhodnou distribuci ve vnitrobuněčném prostředí. Proces může být efektivně studován pomocí mikrofluorescenčních technik. Článek shrnuje současnou situaci v aplikaci pokročilých mikrofluorescenčních metod v dané oblasti a poskytuje krátký popis oligonukleotidové strategie a možností podpory vnitrobuněčného transportu, technické základy fluorescenčního zobrazování a fluorescenční mikrospektroskopie včetně časově rozlišených měření. (cs)
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| Title
| - Advanced Microfluorescence Methods in Monitoring Intracellular Uptake of
- Pokročilé fluorescenční metody pro monitorování průniku antisensních oligonukleotidů do buněk (cs)
- Advanced Microfluorescence Methods in Monitoring Intracellular Uptake of (en)
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| skos:prefLabel
| - Advanced Microfluorescence Methods in Monitoring Intracellular Uptake of
- Pokročilé fluorescenční metody pro monitorování průniku antisensních oligonukleotidů do buněk (cs)
- Advanced Microfluorescence Methods in Monitoring Intracellular Uptake of (en)
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| skos:notation
| - RIV/00216208:11320/07:00004084!RIV08-MSM-11320___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11320/07:00004084
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Advanced; Microfluorescence; Methods; Monitoring; Intracellular; Uptake; of (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Current Organic Chemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kočišová, Eva
- Praus, Petr
- Štěpánek, Josef
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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