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| rdf:type
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| rdfs:seeAlso
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| Description
| - Different primer systems have been developed to characterize arbuscular mycorrhizal fungal (AMF) communities; however, a direct comparison of their specificity, potential to describe diversity and representation of different phylogenetic lineages is lacking. Using seven root samples, we compared four routinely used AMF-specific primer systems for nuclear ribosomal DNA covering i) the partial small subunit (SSU), ii) the partial large subunit (LSU), iii) the partial SSU and internal transcribed spacer (ITS; %22Redecker%22) and iv) the partial SSU ITS partial LSU region (%22Kruger%22). In addition, a new primer combination v) covering the ITS2 region (ITS2) was included in the comparison. The %22Kruger%22 primers tended to yield the highest AMF diversity and showed a significantly higher Shannon diversity index than the SSU primers. We found a strong bias towards the Glomeraceae in the LSU and SSU primer systems and differences in the composition of AMF communities based on the %22Redecker%22 primer system. Our results confirm the crucial role of the choice of target rRNA marker region for analysing AMF communities. We also provide evidence that nested-PCR based data can be interpreted semi-quantitatively and that the extent of observed AMF community overdominance largely depends on the choice of primer.
- Different primer systems have been developed to characterize arbuscular mycorrhizal fungal (AMF) communities; however, a direct comparison of their specificity, potential to describe diversity and representation of different phylogenetic lineages is lacking. Using seven root samples, we compared four routinely used AMF-specific primer systems for nuclear ribosomal DNA covering i) the partial small subunit (SSU), ii) the partial large subunit (LSU), iii) the partial SSU and internal transcribed spacer (ITS; %22Redecker%22) and iv) the partial SSU ITS partial LSU region (%22Kruger%22). In addition, a new primer combination v) covering the ITS2 region (ITS2) was included in the comparison. The %22Kruger%22 primers tended to yield the highest AMF diversity and showed a significantly higher Shannon diversity index than the SSU primers. We found a strong bias towards the Glomeraceae in the LSU and SSU primer systems and differences in the composition of AMF communities based on the %22Redecker%22 primer system. Our results confirm the crucial role of the choice of target rRNA marker region for analysing AMF communities. We also provide evidence that nested-PCR based data can be interpreted semi-quantitatively and that the extent of observed AMF community overdominance largely depends on the choice of primer. (en)
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| Title
| - Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?
- Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution? (en)
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| skos:prefLabel
| - Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?
- Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution? (en)
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| skos:notation
| - RIV/00216208:11310/14:10293606!RIV15-MSM-11310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(GAP504/10/0781), P(GAP504/10/1486), P(GAP505/11/1112), S
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/14:10293606
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - 454-Sequencing; Diversity; Arbuscular mycorrhizal fungi; rRNA; Primers; Glomeromycota (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Soil Biology and Biochemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hejda, Martin
- Kohout, Petr
- Štajerová, Kateřina
- Vosatka, Miroslav
- Sudova, Radka
- Ctvrtlikova, Martina
- Janouskova, Martina
- Pankova, Hana
- Slavikova, Renata
- Sykorova, Zuzana
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1016/j.soilbio.2013.08.027
|
| http://localhost/t...ganizacniJednotka
| |
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