About: DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)

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About: DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1017/S0007485314000224
Description	Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schonherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database. Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schonherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database. (en)
Title	DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) (en)
skos:prefLabel	DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) (en)
skos:notation	RIV/00216208:11310/14:10290813!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LH12160)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Varadínová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	11991
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10290813
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	COI; DNA barcoding; identification; stored-product pests; Cryptolestes (en)
http://linked.open.../riv/klicoveSlovo	Cryptolestes DNA barcoding stored-product pests identification COI
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[148694EE01A8]
http://linked.open...i/riv/nazevZdroje	Bulletin of Entomological Research
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Species diversity of stored grain pests and their biological control using Cheyletus predatory mites
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	104
http://linked.open...iv/tvurceVysledku	Kučerová, Z. Opit, G. Stejskal, V. Cao, Y. Varadínová, Zuzana Li, Z. H. Jiang, F. Li, F. J. Wang, Y. J. Zhang, S. F.
http://linked.open...ain/vavai/riv/wos	000342200200014
issn	0007-4853
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1017/S0007485314000224
http://localhost/t...ganizacniJednotka	11310

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