About: Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

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About: Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/s10535-014-0448-z
Description	We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail moment value, was observed in the inoculated leaves and decreased in the 1(st) to 4(th) systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rate of somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi. We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail moment value, was observed in the inoculated leaves and decreased in the 1(st) to 4(th) systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rate of somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi. (en)
Title	Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi (en)
skos:prefLabel	Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi (en)
skos:notation	RIV/00216208:11310/14:10289034!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GAP501/12/1761), S
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vaculík, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	38082
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10289034
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	somatic mutations; single cell gel electrophoresis; ethyl methanesulphonate; Comet assay (en)
http://linked.open.../riv/klicoveSlovo	Comet assay ethyl methanesulphonate somatic mutations single cell gel electrophoresis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[8E86BC8738DD]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Vaculík, Petr Moravec, Tomáš Plchová, Helena Čeřovská, Noemi Gichner, Tomáš
http://linked.open...ain/vavai/riv/wos	000344596800023
issn	0006-3134
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10535-014-0448-z
http://localhost/t...ganizacniJednotka	11310

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