About: The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal

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About: The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.virol.2013.12.010
Description	Mouse polyomavirus (MPyV) is considered a potential tool for the application of gene therapy; however, the current knowledge of the encapsulation of DNA into virions is vague. We used a series of assays based on the encapsidation of a reporter vector into MPyV pseudovirions to identify putative cis-acting elements that are involved in DNA encapsidation. None of the sequences that were derived from MPyV have been shown to solely enhance the encapsidation of a reporter vector in the assay. The frequency of encapsidation strongly correlated with the total intracellular amount of the vector after transfection. The encapsidation of target DNA into the pseudovirions was shown to be non-specific, and the packaging of non-replicated DNA was observed. We propose that the actual concentration of target DNA at the sites of virion formation is the primary factor that determines its selection for encapsidation. Mouse polyomavirus (MPyV) is considered a potential tool for the application of gene therapy; however, the current knowledge of the encapsulation of DNA into virions is vague. We used a series of assays based on the encapsidation of a reporter vector into MPyV pseudovirions to identify putative cis-acting elements that are involved in DNA encapsidation. None of the sequences that were derived from MPyV have been shown to solely enhance the encapsidation of a reporter vector in the assay. The frequency of encapsidation strongly correlated with the total intracellular amount of the vector after transfection. The encapsidation of target DNA into the pseudovirions was shown to be non-specific, and the packaging of non-replicated DNA was observed. We propose that the actual concentration of target DNA at the sites of virion formation is the primary factor that determines its selection for encapsidation. (en)
Title	The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal (en)
skos:prefLabel	The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal The encapsidation of polyomavirus is not defined by a sequence-specific encapsidation signal (en)
skos:notation	RIV/00216208:11310/14:10288196!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA13-26115S), P(TA03010700), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	neuveden
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Soukup, Jakub Suchanová, Jiřina Španielová, Hana Forstová, Jitka Fraiberk, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	14501
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10288196
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Packaging; Pseudovirion; Assembly; Encapsidation signal; Mouse polyomavirus (en)
http://linked.open.../riv/klicoveSlovo	Encapsidation signal Pseudovirion Assembly Mouse polyomavirus Packaging
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[464C0B347450]
http://linked.open...i/riv/nazevZdroje	Virology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of diagnostic components and adaptable technology of preparation of recombinant vaccines based on capsid nanostructures of the mouse polyomavirus. Doprava genomů polyomavirů do buněčného jádra a interakce strukturních proteinů v hostitelských buňkách
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	450
http://linked.open...iv/tvurceVysledku	Forstová, Jitka Fraiberk, Martin Suchanová, Jiřina Španielová, Hana Soukup, Jakub
http://linked.open...ain/vavai/riv/wos	000331485500014
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0042-6822
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.virol.2013.12.010
http://localhost/t...ganizacniJednotka	11310

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