About: Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1177/1087057113497400
Description	Production of small RNAs by ribonuclease III Dicer is a key step in microRNA and RNA interference pathways, which employ Dicer-produced small RNAs as sequence-specific silencing guides. Further studies and manipulations of microRNA and RNA interference pathways would benefit from identification of small-molecule modulators. Here, we report a study of a fluorescence-based in vitro Dicer cleavage assay, which was adapted for high-throughput screening. The kinetic assay can be performed under single-turnover conditions (35 nM substrate and 70 nM Dicer) in a small volume (5 mu L), which makes it suitable for high-throughput screening in a 1536-well format. As a proof of principle, a small library of bioactive compounds was analyzed, demonstrating potential of the assay. Production of small RNAs by ribonuclease III Dicer is a key step in microRNA and RNA interference pathways, which employ Dicer-produced small RNAs as sequence-specific silencing guides. Further studies and manipulations of microRNA and RNA interference pathways would benefit from identification of small-molecule modulators. Here, we report a study of a fluorescence-based in vitro Dicer cleavage assay, which was adapted for high-throughput screening. The kinetic assay can be performed under single-turnover conditions (35 nM substrate and 70 nM Dicer) in a small volume (5 mu L), which makes it suitable for high-throughput screening in a 1536-well format. As a proof of principle, a small library of bioactive compounds was analyzed, demonstrating potential of the assay. (en)
Title	Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity (en)
skos:prefLabel	Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity Fluorescence-Based High-Throughput Screening of Dicer Cleavage Activity (en)
skos:notation	RIV/00216208:11310/14:10285614!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GA13-29531S), P(LC06077), P(LM2011022), S
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Podolská, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	17096
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10285614
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	high-throughput screening; siRNA; Dicer (en)
http://linked.open.../riv/klicoveSlovo	Dicer siRNA high-throughput screening
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[51CA6D09B8DE]
http://linked.open...i/riv/nazevZdroje	Journal of Biomolecular Screening
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	CZ-OPENSCREEN: National Infrastructure for Chemical Biology Center of Chemical Genetics Vývoj chemických regulátorů mechanismů mikroRNA a RNAi
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Bartůněk, Petr Svoboda, Petr Sedlák, David Bruštíková, Kateřina
http://linked.open...ain/vavai/riv/wos	000330756600009
issn	1087-0571
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1177/1087057113497400
http://localhost/t...ganizacniJednotka	11310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software