About: Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis     Goto   Sponge   NotDistinct   Permalink

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Description
  • A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mgL(-1) for glucose to 0.85 mgL(-1) for lactate.
  • A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mgL(-1) for glucose to 0.85 mgL(-1) for lactate. (en)
Title
  • Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
  • Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis (en)
skos:prefLabel
  • Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
  • Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis (en)
skos:notation
  • RIV/00216208:11310/14:10282018!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, S
http://linked.open...iv/cisloPeriodika
  • September
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 11651
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/14:10282018
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Trimethylsilylation; Protozoa; Metabolites; GC-MS/MS analysis; Aqueous samples (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [1CB05CFBF313]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 967
http://linked.open...iv/tvurceVysledku
  • Čabala, Radomír
  • Gnipova, Anna
  • Horvath, Anton
  • Podolec, Peter
  • Szabo, Alexandra Hengerics
  • Hložek, Tomáš
  • Bierhanzl, Václav
  • Blasko, Jaroslav
  • Gorova, Renata
  • Kubinec, Robert
  • Visnovsky, Jozef
http://linked.open...ain/vavai/riv/wos
  • 000341468600020
issn
  • 1570-0232
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.jchromb.2014.07.023
http://localhost/t...ganizacniJednotka
  • 11310
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