About: Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.clinbiochem.2014.06.076
Description	Objectives: The aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC-FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes. Design and methods: 100 mu L of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC-MS. Results: The assay was linear in the concentration range of 10-400 mu gmL(-1), with adequate accuracy and precision for GC-FID (98-106.7%, CV {= 9.1%, respectively) and for GC-MS (99.3-105.5%, CV {= 9.2%, respectively). Conclusion: The entire sample preparation procedure is completed within 5 min and the quantitative results are available within 35 min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. Objectives: The aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC-FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes. Design and methods: 100 mu L of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC-MS. Results: The assay was linear in the concentration range of 10-400 mu gmL(-1), with adequate accuracy and precision for GC-FID (98-106.7%, CV {= 9.1%, respectively) and for GC-MS (99.3-105.5%, CV {= 9.2%, respectively). Conclusion: The entire sample preparation procedure is completed within 5 min and the quantitative results are available within 35 min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. (en)
Title	Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID (en)
skos:prefLabel	Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID (en)
skos:notation	RIV/00216208:11310/14:10282015!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	15
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Čabala, Radomír Bursová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	16502
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10282015
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GC; Experimental design; Chloroformate; Non-steroidal anti-inflammatory drugs (en)
http://linked.open.../riv/klicoveSlovo	GC Experimental design Chloroformate Non-steroidal anti-inflammatory drugs
http://linked.open...odStatuVydavatele	CA - Kanada
http://linked.open...ontrolniKodProRIV	[9EBFC46FA996]
http://linked.open...i/riv/nazevZdroje	Clinical Biochemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Čabala, Radomír Bursová, Miroslava Hložek, Tomáš
http://linked.open...ain/vavai/riv/wos	000342822100018
issn	0009-9120
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.clinbiochem.2014.06.076
http://localhost/t...ganizacniJednotka	11310

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