About: 7-Aryl-7-deazaadenine 2 '-Deoxyribonucleoside Triphosphates (dNTPs): Better Substrates for DNA Polymerases than dATP in Competitive Incorporations     Goto   Sponge   NotDistinct   Permalink

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Description
  • A series of 7-substituted 7-deazaadenine and 5-substituted cytosine 2'-deoxyribonucleoside triphosphates (dNTPs) were tested for their competitive incorporations (in the presence of dATP and dCTP) into DNA by several DNA polymerases by using analysis based on cleavage by restriction endonucleases. 7-Aryl-7-deazaadenine dNTPs were more efficient substrates than dATP because of their higher affinity for the active site of the enzyme, as proved by kinetic measurements and calculations.
  • A series of 7-substituted 7-deazaadenine and 5-substituted cytosine 2'-deoxyribonucleoside triphosphates (dNTPs) were tested for their competitive incorporations (in the presence of dATP and dCTP) into DNA by several DNA polymerases by using analysis based on cleavage by restriction endonucleases. 7-Aryl-7-deazaadenine dNTPs were more efficient substrates than dATP because of their higher affinity for the active site of the enzyme, as proved by kinetic measurements and calculations. (en)
Title
  • 7-Aryl-7-deazaadenine 2 '-Deoxyribonucleoside Triphosphates (dNTPs): Better Substrates for DNA Polymerases than dATP in Competitive Incorporations
  • 7-Aryl-7-deazaadenine 2 '-Deoxyribonucleoside Triphosphates (dNTPs): Better Substrates for DNA Polymerases than dATP in Competitive Incorporations (en)
skos:prefLabel
  • 7-Aryl-7-deazaadenine 2 '-Deoxyribonucleoside Triphosphates (dNTPs): Better Substrates for DNA Polymerases than dATP in Competitive Incorporations
  • 7-Aryl-7-deazaadenine 2 '-Deoxyribonucleoside Triphosphates (dNTPs): Better Substrates for DNA Polymerases than dATP in Competitive Incorporations (en)
skos:notation
  • RIV/00216208:11310/14:10271951!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GBP206/12/G151)
http://linked.open...iv/cisloPeriodika
  • 29
http://linked.open...vai/riv/dodaniDat
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  • 58362
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  • RIV/00216208:11310/14:10271951
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  • nucleotides; nucleoside triphosphates; kinetics; enzymes; DNA (en)
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [4602A9F9CDBA]
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  • Angewandte Chemie - International Edition
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  • 53
http://linked.open...iv/tvurceVysledku
  • Hocek, Michal
  • Fanfrlík, Jindřich
  • Kielkowski, Pavel
http://linked.open...ain/vavai/riv/wos
  • 000339564800025
issn
  • 1433-7851
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/anie.201404742
http://localhost/t...ganizacniJednotka
  • 11310
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