About: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

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About: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://nar.oxfordjournals.org/content/42/8/5151.full.pdf+html
Description	Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery. Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery. (en)
Title	Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis (en)
skos:prefLabel	Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis (en)
skos:notation	RIV/00216208:11310/14:10218613!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0109), P(EE2.3.30.0029), P(GA13-16842S), P(GAP302/11/0855), P(GBP305/12/G034), S
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Korelusová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	6940
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10218613
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	expression; holoenzyme; purification; RapA; protein; transcription; delta subunit; bacterial homolog; Escherichia coli (en)
http://linked.open.../riv/klicoveSlovo	Escherichia coli delta subunit expression protein transcription RapA bacterial homolog holoenzyme purification
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[330D9DAAFF64]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University RNA polymerase: The “meeting point” of regulatory networks Centre for RNA Biology PODJEDNOTKY URČUJÍCÍ DNA SPECIFICITU BAKTERIÁLNÍ RNA POLYMERÁZY S FLEXIBILNÍMI DOMÉNAMI: FUNKCE A DYNAMIKA BIOPOL: BIOpolymer POstdoc Laboratory and educative center
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Dohnálek, Jan Halada, Petr Kovaľ, Tomáš Krásný, Libor Šanderová, Hana Rabatinová, Alžběta Rittich, Šimon Fu, Zhihui Lewis, Peter Ramaniuk, Olga Sudzinová, Petra Wiedermannová, Jana
http://linked.open...ain/vavai/riv/wos	000336092300042
issn	0305-1048
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/nar/gku113
http://localhost/t...ganizacniJednotka	11310

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