About: Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain

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About: Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.electrochemsci.org/papers/vol8/80100125.pdf
Description	To study the enigmatic role of cytochrome b(5) in a cytochrome P450 monooxygenase multienzyme system, we expressed and purified a photoreactive analogue of cytochrome b(5), having methionine residues substituted with photolabile diazirine derivatives (photo-cyt b(5)). An equimolar mixture of cytochrome P450 (CYP) 2B4 and photo-cyt b(5) was photolyzed and products separated using SDS-PAGE. Several oligomers composed of CYP2B4 and cyt b(5) (MALDI-TOF/TOF mass spectrometry) in 1: 1, 1: 2, and 2: 1 molar ratios have been found and their covalent nature confirmed by MALDI-TOF/TOF mass spectrometry of chymotryptic peptides. The amount of oligomers formed was markedly increased by the presence of the CYP2B4 substrate, diamantane, showing that the substrate binding increases the affinity between cytochromes P450 and b(5). To study the enigmatic role of cytochrome b(5) in a cytochrome P450 monooxygenase multienzyme system, we expressed and purified a photoreactive analogue of cytochrome b(5), having methionine residues substituted with photolabile diazirine derivatives (photo-cyt b(5)). An equimolar mixture of cytochrome P450 (CYP) 2B4 and photo-cyt b(5) was photolyzed and products separated using SDS-PAGE. Several oligomers composed of CYP2B4 and cyt b(5) (MALDI-TOF/TOF mass spectrometry) in 1: 1, 1: 2, and 2: 1 molar ratios have been found and their covalent nature confirmed by MALDI-TOF/TOF mass spectrometry of chymotryptic peptides. The amount of oligomers formed was markedly increased by the presence of the CYP2B4 substrate, diamantane, showing that the substrate binding increases the affinity between cytochromes P450 and b(5). (en)
Title	Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain (en)
skos:prefLabel	Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain (en)
skos:notation	RIV/00216208:11310/13:10134468!RIV14-GA0-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GA303/09/0472), P(GAP207/12/0627), P(GAP301/10/0356), P(GD305/09/H008), S
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Koberová, Monika Kotrbová, Věra Hodek, Petr Ječmen, Tomáš Hudeček, Jiří Stiborová, Marie Šulc, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	96087
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/13:10134468
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cross-linking; Mass Spectromery; Cytochrome P450 2B4; Cytochrome b(5); Photo-methionine; Electron-transport chain (en)
http://linked.open.../riv/klicoveSlovo	Cytochrome P450 2B4 Cytochrome b(5) Electron-transport chain Mass Spectromery Photo-methionine Cross-linking
http://linked.open...odStatuVydavatele	RS - Srbská republika
http://linked.open...ontrolniKodProRIV	[FD6D411390F3]
http://linked.open...i/riv/nazevZdroje	International Journal of Electrochemical Science
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Study on participation of biotransformation enzymes in development of renal injury and urothelial cancer mediated by aristolochic acid Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects INTERAKCE SAVČÍHO MIKROSOMÁLNÍHO CYTOCHROMU P450 S REDOX PARTNERY - TOPOLOGIE A STRUKTURNĚ-FUNKČNÍ VZTAHY
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Hodek, Petr Hudeček, Jiří Ječmen, Tomáš Kizek, René Stiborová, Marie Šulc, Miroslav Černá, Věra Koberová, Monika
http://linked.open...ain/vavai/riv/wos	000316562800010
issn	1452-3981
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	11310

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