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  • Chicken antibodies (IgY) are increasingly being used as a primary antibody in the Western blotting immunodetection, offering several advantages compared to the common mammalian antibodies. The presence of additional bands in the region of 45-70 kDa, vertical streaks, and spots on the membrane, caused by human keratins, made the evaluation of the developed blots hardly possible. In this study, we describe a method how to eliminate the cross-reactivity of IgY with human keratins.
  • Chicken antibodies (IgY) are increasingly being used as a primary antibody in the Western blotting immunodetection, offering several advantages compared to the common mammalian antibodies. The presence of additional bands in the region of 45-70 kDa, vertical streaks, and spots on the membrane, caused by human keratins, made the evaluation of the developed blots hardly possible. In this study, we describe a method how to eliminate the cross-reactivity of IgY with human keratins. (en)
Title
  • Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It
  • Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It (en)
skos:prefLabel
  • Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It
  • Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It (en)
skos:notation
  • RIV/00216208:11310/13:10133399!RIV14-GA0-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GBP303/12/G163)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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  • 82601
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/13:10133399
http://linked.open...riv/jazykVysledku
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  • Keratinocytes; Keratin-interference; Immuno-detection; Western Blots; Electrophoresis; Cytochrome P450 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • RS - Srbská republika
http://linked.open...ontrolniKodProRIV
  • [2391E4863AEA]
http://linked.open...i/riv/nazevZdroje
  • International Journal of Electrochemical Science
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  • 8
http://linked.open...iv/tvurceVysledku
  • Hodek, Petr
  • Stiborová, Marie
  • Šulc, Miroslav
  • Křížková, Jitka
  • Franková, Jana
  • Mácová, Iva
http://linked.open...ain/vavai/riv/wos
  • 000316565800001
issn
  • 1452-3981
number of pages
http://localhost/t...ganizacniJednotka
  • 11310
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