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Description
  • Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46 kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed.
  • Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46 kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed. (en)
Title
  • Individual variability of salivary gland proteins in three Phlebotomus species
  • Individual variability of salivary gland proteins in three Phlebotomus species (en)
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  • Individual variability of salivary gland proteins in three Phlebotomus species
  • Individual variability of salivary gland proteins in three Phlebotomus species (en)
skos:notation
  • RIV/00216208:11310/12:10127523!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(7E11055), P(GA206/09/0777), P(GA206/09/0822), P(LC06009), S, Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 141220
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/12:10127523
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Anti-Leishmania vaccine; Leishmania transmission; Individual variability; Salivary glands; Phlebotomine sand flies; Phlebotomus (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [BD4E9CC5CC75]
http://linked.open...i/riv/nazevZdroje
  • Acta Tropica
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http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 122
http://linked.open...iv/tvurceVysledku
  • Rohoušová, Iva
  • Volf, Petr
  • Volfová, Věra
  • Nová, Soňa
http://linked.open...ain/vavai/riv/wos
  • 000302668600012
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-706X
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.actatropica.2011.12.004
http://localhost/t...ganizacniJednotka
  • 11310
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