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| Description
| - A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons.
- A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons. (en)
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| Title
| - Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode
- Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode (en)
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| skos:prefLabel
| - Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode
- Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode (en)
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| skos:notation
| - RIV/00216208:11310/12:10126406!RIV13-GA0-11310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(GBP206/12/G151), P(ME10004), S, Z(MSM0021620857)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/12:10126406
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Human urine; Solid-phase extraction; Boron-doped diamond film electrode; High-performance liquid chromatography with electrochemical detection; 1-Hydroxypyrene (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Analytical and Bioanalytical Chemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Barek, Jiří
- Vyskočil, Vlastimil
- Yosypchuk, Oksana
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1007/s00216-012-6140-2
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| http://localhost/t...ganizacniJednotka
| |
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