About: Stability of influenza virus as evaluated by integrity of its RNA

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.4149/av_2012_02_125
Description	Various methods of handling samples of avian influenza prior to detecting influenza viruses can significantly influence both, the detection of the virus and the quantification of viral nucleic acids. The quantity of influenza viral RNA remaining in different collecting buffers and kept at temperatures of -20 degrees C, +4 degrees C or +22 degrees C for various lengths of time, was determined. The quantity of viral RNA remained the same for 120 days at -20 degrees C, but decreased when the samples were stored at either +4 degrees C or +22 degrees C. The quantity of RNA was influenced by the composition of the collecting buffer. The influenza virus sample that is to be used for RNA quantification can be stored at +4 degrees C and freeze and thaw cycles should be avoided during transport. Our results clearly indicate that the quality and quantity of influenza virus nucleic acid depends on the chemical composition of used buffer and also that the samples can be protected from degradation even if they are not stored at ultra-low temperatures. However, repeated thaw and freeze cycles will damage viral RNA even if kept in stabilizing buffers. Various methods of handling samples of avian influenza prior to detecting influenza viruses can significantly influence both, the detection of the virus and the quantification of viral nucleic acids. The quantity of influenza viral RNA remaining in different collecting buffers and kept at temperatures of -20 degrees C, +4 degrees C or +22 degrees C for various lengths of time, was determined. The quantity of viral RNA remained the same for 120 days at -20 degrees C, but decreased when the samples were stored at either +4 degrees C or +22 degrees C. The quantity of RNA was influenced by the composition of the collecting buffer. The influenza virus sample that is to be used for RNA quantification can be stored at +4 degrees C and freeze and thaw cycles should be avoided during transport. Our results clearly indicate that the quality and quantity of influenza virus nucleic acid depends on the chemical composition of used buffer and also that the samples can be protected from degradation even if they are not stored at ultra-low temperatures. However, repeated thaw and freeze cycles will damage viral RNA even if kept in stabilizing buffers. (en)
Title	Stability of influenza virus as evaluated by integrity of its RNA Stability of influenza virus as evaluated by integrity of its RNA (en)
skos:prefLabel	Stability of influenza virus as evaluated by integrity of its RNA Stability of influenza virus as evaluated by integrity of its RNA (en)
skos:notation	RIV/00216208:11310/12:10125432!RIV13-MSM-11310___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(SP/2D3/60/08), Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Stopka, Pavel Ryba, Štěpán
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	170820
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10125432
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	influenza virus, degradation, RNA, buffer; REAGENT; RECOVERY; COLLECTION; RIBONUCLEASE; PCR; A VIRUSES; AMMONIUM-SULFATE; ROOM-TEMPERATURE (en)
http://linked.open.../riv/klicoveSlovo	degradation buffer PCR RNA influenza virus RECOVERY ROOM-TEMPERATURE A VIRUSES AMMONIUM-SULFATE COLLECTION REAGENT RIBONUCLEASE
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[6866C80F864F]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated research of the effects and consequences of artificially reared mallards (Anas platyrhynchos) on wetland ecosystems with the application of advanced ecological and molecular techniques.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Stopka, Pavel Ryba, Štěpán Havlickova, M. Tacner, J.
http://linked.open...ain/vavai/riv/wos	000307860400005
http://linked.open...n/vavai/riv/zamer	Ekologické procesy v evoluci modelových skupin organismů
issn	0001-723X
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.4149/av_2012_02_125
http://localhost/t...ganizacniJednotka	11310

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