About: Production of Aspergillus niger beta-mannosidase in Pichia pastoris

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About: Production of Aspergillus niger beta-mannosidase in Pichia pastoris Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.pep.2012.07.012
Description	beta-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various sugar chains. cDNA corresponding to the beta-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The beta-mannosidase gene contains an open reading frame which encodes the protein with 933 amino acid residues. The wild type and recombinant proteins were purified to apparent homogeneity and biochemically characterized (K-M 0.28 and 0.44 mmol/l for p-nitrophenyl beta-D-mannopyranoside, pI 4.2 and 4.0, and their pH optima were at pH 4.5 and 5.5 and 65 degrees C, respectively). beta-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various sugar chains. cDNA corresponding to the beta-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The beta-mannosidase gene contains an open reading frame which encodes the protein with 933 amino acid residues. The wild type and recombinant proteins were purified to apparent homogeneity and biochemically characterized (K-M 0.28 and 0.44 mmol/l for p-nitrophenyl beta-D-mannopyranoside, pI 4.2 and 4.0, and their pH optima were at pH 4.5 and 5.5 and 65 degrees C, respectively). (en)
Title	Production of Aspergillus niger beta-mannosidase in Pichia pastoris Production of Aspergillus niger beta-mannosidase in Pichia pastoris (en)
skos:prefLabel	Production of Aspergillus niger beta-mannosidase in Pichia pastoris Production of Aspergillus niger beta-mannosidase in Pichia pastoris (en)
skos:notation	RIV/00216208:11310/12:10125394!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(7E11011), P(GAP207/10/0321), P(GD305/09/H008), S
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Weignerová, Lenka Fliedrová, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	162456
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10125394
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	beta-mannosidase, aspergillus niger, cloning, expression; gene; alkyl; reesei; binding; aculeatus; expression; purification; overexpression; cloning; Mannan-degrading enzymes (en)
http://linked.open.../riv/klicoveSlovo	expression gene Mannan-degrading enzymes aculeatus alkyl aspergillus niger beta-mannosidase overexpression reesei binding cloning purification
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[030EFCB39A3D]
http://linked.open...i/riv/nazevZdroje	Protein Expression and Purification
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects Novel Biocatalysts for the Production of Glycosides The ways to N-acetyl-mannosamine structures via mannoside metabolizing enzymes
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	85
http://linked.open...iv/tvurceVysledku	Křen, Vladimír Weignerová, Lenka Fliedrová, Barbora Gerstorferová, Daniela
http://linked.open...ain/vavai/riv/wos	000309786600001
issn	1046-5928
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.pep.2012.07.012
http://localhost/t...ganizacniJednotka	11310

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