About: Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.bpj.2012.09.021
Description	Phosducin (Pdc), a highly conserved phosphoprotein, plays an important role in the regulation of G protein signaling, transcriptional control, and modulation of blood pressure. Pdc is negatively regulated by phosphorylation followed by binding to the 14-3-3 protein, whose role is still unclear. To gain insight into the role of 14-3-3 in the regulation of Pdc function, we studied structural changes of Pdc induced by phosphorylation and 14-3-3 protein binding using time-resolved fluorescence spectroscopy. Our data show that the phosphorylation of the N-terminal domain of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule. Complex formation with 14-3-3 reduces the flexibility of both the N- and C-terminal domains of phosphorylated Pdc, as determined by time-resolved tryptophan and dansyl fluorescence Phosducin (Pdc), a highly conserved phosphoprotein, plays an important role in the regulation of G protein signaling, transcriptional control, and modulation of blood pressure. Pdc is negatively regulated by phosphorylation followed by binding to the 14-3-3 protein, whose role is still unclear. To gain insight into the role of 14-3-3 in the regulation of Pdc function, we studied structural changes of Pdc induced by phosphorylation and 14-3-3 protein binding using time-resolved fluorescence spectroscopy. Our data show that the phosphorylation of the N-terminal domain of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule. Complex formation with 14-3-3 reduces the flexibility of both the N- and C-terminal domains of phosphorylated Pdc, as determined by time-resolved tryptophan and dansyl fluorescence (en)
Title	Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding (en)
skos:prefLabel	Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding (en)
skos:notation	RIV/00216208:11310/12:10124478!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GAP305/11/0708), S, Z(MSM0021620857)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Obšil, Tomáš Šulc, Miroslav Štěpánek, Miroslav Řežábková, Lenka Kacířová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	171832
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10124478
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence; 14-3-3 protein; phosducin (en)
http://linked.open.../riv/klicoveSlovo	fluorescence 14-3-3 protein phosducin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[84BC64C02E79]
http://linked.open...i/riv/nazevZdroje	Biophysical Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of the 14-3-3 protein in the regulation of phosducin function
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	103
http://linked.open...iv/tvurceVysledku	Heřman, Petr Obšil, Tomáš Šulc, Miroslav Štěpánek, Miroslav Večeř, Jaroslav Obšilová, Veronika Řežábková, Lenka Kacířová, Miroslava
http://linked.open...ain/vavai/riv/wos	000310785300023
http://linked.open...n/vavai/riv/zamer	Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
issn	0006-3495
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bpj.2012.09.021
http://localhost/t...ganizacniJednotka	11310

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