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Description
  • New colorimetric methods are described for determination of sub-milligram amounts of ultra-high molecular weight polyethylene (UHMWPE) wear particles. These methods are based on the irreversible binding of the fluorescein-conjugated bovine serum albumin or the hydrophobic dye Oil Red O to wear particles. UHMWPE particles bind both substances from their solutions and thus decrease the absorbance of these solutions. The decrease is linearly dependent on the amount of added wear particles in the sub-milligram range suitable for practical use. The newly developed method offers improved accuracy and precision compared to Fourier transformed infrared spectroscopy (Slouf M. et al. Quantification of UHMWPE wear in periprosthetic tissues of hip arthoplasty: description of a new method based on IR and comparison with radiographic appearance.
  • New colorimetric methods are described for determination of sub-milligram amounts of ultra-high molecular weight polyethylene (UHMWPE) wear particles. These methods are based on the irreversible binding of the fluorescein-conjugated bovine serum albumin or the hydrophobic dye Oil Red O to wear particles. UHMWPE particles bind both substances from their solutions and thus decrease the absorbance of these solutions. The decrease is linearly dependent on the amount of added wear particles in the sub-milligram range suitable for practical use. The newly developed method offers improved accuracy and precision compared to Fourier transformed infrared spectroscopy (Slouf M. et al. Quantification of UHMWPE wear in periprosthetic tissues of hip arthoplasty: description of a new method based on IR and comparison with radiographic appearance. (en)
Title
  • Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles
  • Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles (en)
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  • Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles
  • Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles (en)
skos:notation
  • RIV/00216208:11310/12:10120333!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(2B06096), P(GAP503/11/0163), P(NT12229), Z(AV0Z40500505), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 167892
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/12:10120333
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • number; failure; quantification; oxidation; debris; joint replacements; periprosthetic tissues; hip-arthroplasty; UHMWPE wear; colorimetric methods; wear particles determination; polyethylene wear particles; joint replacement (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [38BC569F90A5]
http://linked.open...i/riv/nazevZdroje
  • Acta Biomaterialia
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 8
http://linked.open...iv/tvurceVysledku
  • Šlouf, Miroslav
  • Kaftan, Filip
  • Entlicher, Gustav
  • Veselý, Filip
  • Zolotarevová, Eva
  • Špundová, Marcela
http://linked.open...ain/vavai/riv/wos
  • 000302989700028
http://linked.open...n/vavai/riv/zamer
issn
  • 1742-7061
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.actbio.2012.01.010
http://localhost/t...ganizacniJednotka
  • 11310
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