About: Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036314
Description	Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. (en)
Title	Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology (en)
skos:prefLabel	Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology (en)
skos:notation	RIV/00216208:11310/12:10119138!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP305/10/0651), P(LC07032), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Doležal, Pavel Tachezy, Jan Voleman, Luboš Marcinčiková, Michaela Martincová, Eva Najdrová, Vladimíra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	147267
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10119138
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	organelles; Giardia intestinalis; Trichomonas vaginalis; HaloTag; Hydrogenosomes; Mitosomes (en)
http://linked.open.../riv/klicoveSlovo	Mitosomes Trichomonas vaginalis Hydrogenosomes HaloTag organelles Giardia intestinalis
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[881E85E8A9AF]
http://linked.open...i/riv/nazevZdroje	PLoS ONE
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	14 (xsd:int)
http://linked.open...vavai/riv/projekt	The evolution of mitochondrial biogenesis. Center for Functional Genetics
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Doležal, Pavel Tachezy, Jan Marcinčiková, Michaela Voleman, Luboš De Napoli, Maximiliano Eshar, Shiri Gualdron, Melisa Hopp, Christine S. Martincová, Eva Najdrová, Vladimíra Sanin, David E. Tembo, Dumizulu L. Van Tyne, Daria Walker, Dawn
http://linked.open...ain/vavai/riv/wos	000305336000188
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	1932-6203
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0036314
http://localhost/t...ganizacniJednotka	11310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software