About: Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0166685111002179
Description	Trichomonas vaginalis has been reported to possess alternative 2-keto acid oxidoreductases (KORs). These enzymes preferentially used indolepyruvate in a reaction that resembled that of pyruvate:ferredoxin oxidoreductase (PFO). However, the KORs did not reduce ferredoxin and remained active in metronidazole-resistant trichomonads lacking PFO. Therefore, it was proposed that the KORs may help trichomonads to survive in the presence of metronidazole. The KORs were identified using activity staining on native gels (Brown DM, Upcroft JA, Dodd HN, et al. Alternative 2-keto acid oxidoreductase activities in T. vaginalis. Mol Biochem Parasitol 1999;98:203-14). In the current study, we showed that the apparent KOR activity was caused by the non-enzymatic reduction of the indicator dye, nitroblue tetrazolium, by indolepyruvate, which is facilitated by Triton X-100 used to prepare the membrane fractions. We could not confirm the presence of KORs in metronidazole-resistant T. vaginalis. Trichomonas vaginalis has been reported to possess alternative 2-keto acid oxidoreductases (KORs). These enzymes preferentially used indolepyruvate in a reaction that resembled that of pyruvate:ferredoxin oxidoreductase (PFO). However, the KORs did not reduce ferredoxin and remained active in metronidazole-resistant trichomonads lacking PFO. Therefore, it was proposed that the KORs may help trichomonads to survive in the presence of metronidazole. The KORs were identified using activity staining on native gels (Brown DM, Upcroft JA, Dodd HN, et al. Alternative 2-keto acid oxidoreductase activities in T. vaginalis. Mol Biochem Parasitol 1999;98:203-14). In the current study, we showed that the apparent KOR activity was caused by the non-enzymatic reduction of the indicator dye, nitroblue tetrazolium, by indolepyruvate, which is facilitated by Triton X-100 used to prepare the membrane fractions. We could not confirm the presence of KORs in metronidazole-resistant T. vaginalis. (en)
Title	Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining (en)
skos:prefLabel	Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining Alternative 2-keto acid oxidoreductases in Trichomonas vaginalis: Artifact of histochemical staining (en)
skos:notation	RIV/00216208:11310/12:10099398!RIV13-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(LC07032), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Hrdý, Ivan Campo Beltran, Neritza Tachezy, Jan Zedníková, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	121764
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10099398
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nitroblue tetrazolium; Metronidazole; Pyruvate:ferredoxin oxidoreductase; Hydrogenosome; Keto acid oxidoreductase; Trichomonas (en)
http://linked.open.../riv/klicoveSlovo	Keto acid oxidoreductase Metronidazole Nitroblue tetrazolium Pyruvate:ferredoxin oxidoreductase Trichomonas Hydrogenosome
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3ED7FEFAA34B]
http://linked.open...i/riv/nazevZdroje	Molecular and Biochemical Parasitology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Functional Genetics
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	181
http://linked.open...iv/tvurceVysledku	Campo Beltran, Neritza Hrdý, Ivan Tachezy, Jan Zedníková, Věra
http://linked.open...ain/vavai/riv/wos	000298828900009
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0166-6851
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.molbiopara.2011.09.005
http://localhost/t...ganizacniJednotka	11310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software