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Description
  • Sand flies used to have a reputation for being difficult and labour-intensive to breed. Here we summarize our experience with establishment and maintenance of sand fly colonies and their use for infective experiments: techniques for collection and handling wild-caught females, rearing larvae and adults and experimental infections of sand flies by Leishmania using membrane feeding. In addition, we compare major life cycle parameters between various colonies maintained under standard laboratory conditions. The sand fly rearing is tricky but some species can be reared in large numbers with a minimum of space and equipment. Initiation of new colonies from endemic sites is a prerequisite for accurate studies on parasite-vector interaction but it is more difficult step than routine maintenance of colonies already established in laboratory for many generations.
  • Sand flies used to have a reputation for being difficult and labour-intensive to breed. Here we summarize our experience with establishment and maintenance of sand fly colonies and their use for infective experiments: techniques for collection and handling wild-caught females, rearing larvae and adults and experimental infections of sand flies by Leishmania using membrane feeding. In addition, we compare major life cycle parameters between various colonies maintained under standard laboratory conditions. The sand fly rearing is tricky but some species can be reared in large numbers with a minimum of space and equipment. Initiation of new colonies from endemic sites is a prerequisite for accurate studies on parasite-vector interaction but it is more difficult step than routine maintenance of colonies already established in laboratory for many generations. (en)
Title
  • Establishment and maintenance of sand fly colonies
  • Establishment and maintenance of sand fly colonies (en)
skos:prefLabel
  • Establishment and maintenance of sand fly colonies
  • Establishment and maintenance of sand fly colonies (en)
skos:notation
  • RIV/00216208:11310/11:10108159!RIV12-MSM-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika
  • 2.3.2011
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 197949
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/11:10108159
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • infectivity; perniciosus; dogs; transmission; flies; diptera psychodidae; leishmania-infantum; Phlebotomine sandflies (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [AA7453E7BB02]
http://linked.open...i/riv/nazevZdroje
  • Journal of Vector Ecology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 36
http://linked.open...iv/tvurceVysledku
  • Volf, Petr
  • Volfová, Věra
http://linked.open...ain/vavai/riv/wos
  • 000287927300001
http://linked.open...n/vavai/riv/zamer
issn
  • 1081-1710
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/j.1948-7134.2011.00106.x
http://localhost/t...ganizacniJednotka
  • 11310
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