About: Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes.

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About: Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1002/jssc.201000869
Description	Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with buffer and protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example. Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with buffer and protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example. (en)
Title	Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. (en)
skos:prefLabel	Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. (en)
skos:notation	RIV/00216208:11310/11:10107525!RIV12-GA0-11310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA303/09/1285)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Tichá, Ivana Tichá, Marie Dráb, Tomáš Liberda, Jiří Kračmerová, Jana Hanzlíková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	215007
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/11:10107525
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	page; form; mannosidase; glucosidase; purification; galactosidase; seeds; Gel-electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	form Gel-electrophoresis galactosidase glucosidase mannosidase page seeds purification
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[2E3E555325E2]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of the sperm membrane proteins with zona pellucida binding activity
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Tichá, Marie Tichá, Ivana Dráb, Tomáš Hanzlíková, Eva Kračmerová, Jana Liberda, Jiří
http://linked.open...ain/vavai/riv/wos	000293588900009
issn	1615-9306
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jssc.201000869
http://localhost/t...ganizacniJednotka	11310

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