About: Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes

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About: Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.molbiopara.2010.12.011
Description	The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome land II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mapping of the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping. The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome land II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mapping of the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping. (en)
Title	Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes (en)
skos:prefLabel	Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes (en)
skos:notation	RIV/00216208:11310/11:10099223!RIV12-MSM-11310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC07032), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Krylov, Vladimír Tachezy, Jan Zubáčová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	200020
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/11:10099223
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	FISH; Chromosome; Trichomonas vaginalis (en)
http://linked.open.../riv/klicoveSlovo	Chromosome Trichomonas vaginalis FISH
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[EEF82A8BBE68]
http://linked.open...i/riv/nazevZdroje	Molecular and Biochemical Parasitology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Functional Genetics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	176
http://linked.open...iv/tvurceVysledku	Krylov, Vladimír Tachezy, Jan Zubáčová, Zuzana
http://linked.open...ain/vavai/riv/wos	000288629800011
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0166-6851
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.molbiopara.2010.12.011
http://localhost/t...ganizacniJednotka	11310

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